Sorption of ferrous iron by EPS from the acidophilic bacterium Acidiphilium Sp.A mechanism proposal

  1. Jaime M. Tapia 1
  2. J. A. Muñoz Sánchez 2
  3. Felisa González González 2
  4. Mª Luisa Blázquez Izquierdo 2
  5. Antonio Ballester Pérez 2
  1. 1 Universidad Arturo Prat, Facultad de Ingeniería y Arquitectura
  2. 2 Universidad Complutense, Departamento de Ciencia de los Materiales e Ingeniería Metalúrgica
Revista de metalurgia

ISSN: 0034-8570

Year of publication: 2016

Volume: 52

Issue: 3

Pages: 76

Type: Article

DOI: 10.3989/REVMETALM.076 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Revista de metalurgia


Cited by

  • Scopus Cited by: 3 (20-11-2023)
  • Web of Science Cited by: 2 (18-10-2023)
  • Dimensions Cited by: 1 (31-03-2023)

JCR (Journal Impact Factor)

  • Year 2016
  • Journal Impact Factor: 0.345
  • Journal Impact Factor without self cites: 0.293
  • Article influence score: 0.091
  • Best Quartile: Q4
  • Area: METALLURGY & METALLURGICAL ENGINEERING Quartile: Q4 Rank in area: 64/74 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2016
  • SJR Journal Impact: 0.159
  • Best Quartile: Q4
  • Area: Physical and Theoretical Chemistry Quartile: Q4 Rank in area: 146/172
  • Area: Materials Chemistry Quartile: Q4 Rank in area: 228/348
  • Area: Metals and Alloys Quartile: Q4 Rank in area: 129/205
  • Area: Condensed Matter Physics Quartile: Q4 Rank in area: 382/491

Scopus CiteScore

  • Year 2016
  • CiteScore of the Journal : 0.6
  • Area: Metals and Alloys Percentile: 32
  • Area: Materials Chemistry Percentile: 20
  • Area: Condensed Matter Physics Percentile: 11
  • Area: Physical and Theoretical Chemistry Percentile: 7


(Data updated as of 31-03-2023)
  • Total citations: 1
  • Recent citations: 0
  • Field Citation Ratio (FCR): 0.11


The aim of this work was to assess the uptake of Fe(II) by extracellular polymeric substances (EPS) from the acidophilic bacterium Acidiphillium 3.2Sup(5). These EPS were extracted using EDTA. EPS of A. 3.2Sup(5) loaded in sorption tests with Fe(II), were characterized using the following experimental techniques: scanning electron microscopy (SEM) with energy dispersive X-ray microanalysis (EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The experimental results indicate that EPS adsorb ferrous iron according to Freundlich model with a metal sorption uptake of K = 1.14 mg1-1/n L1/n g-1 and a sorption intensity of 1/n = 1.26. In addition, ferrous iron sorption by EPS took place by preferential interaction with the carboxyl group which promotes the formation of ferrous iron oxalates (FeC2O4). Since the interaction reaction was reversible (Log K = 0.77 ± 0.33), that means that the cation sorption can be reversed at convenience.

Bibliographic References

  • Casas, J.M., Crisóstomo, G., Cifuentes, L. (2005). Speciation of the Fe(Ii)-Fe(Iii)-H2so4-H2o System at 25 and 50 °C. Hydro- metallurgy 80 (4), 254–264.
  • Dopson, M., Ni, G., Sleutels, T. (2015). Possibilities for extremo- philic microorganisms in microbial electrochemical sys- tems. FEMS Microbiol. Rev. 40 (2), 164–181. PMid:26474966 PMCid:PMC4802824
  • Figueira, M.M., Volesky, B., Mathieu, H.J. (1999). Instrumen- tal analysis study of iron species biosorption by sargassum biomass. Environ. Sci. Technol. 33 (11), 1840–1846.
  • Fuks, L., Filipiuk, D., Majdan, M. (2006). Transition metal complexes with alginate biosorbent. J. Mol. Struct. 792–793, 104–109.
  • Guibaud, G., Hullebusch, E.V., Bordas, F., d'Abzac, P., Joussein, E. (2009). Sorption of Cd(Ii) and Pb(Ii) by Exopolymeric Substances (Eps) Extracted from activated sludges and pure bacterial strains: Modelling of the Metal/Ligand ratio effect and role of the mineral fraction. Bioresource Technol. 100 (12), 2959–2968. PMid:19254840
  • Kareem, S., Adeogun, A., Omeike, S. (2014). Biosorption Studies for the Removal of Ferrous Ion from Aqueous Solution by Aspergillus Terreus and Trichoderma Viride: Kinetic, Thermodynamic and Isothermal Parameters. J. Water Supply Res Tech-Aqua 63 (1), 66–75.
  • Konhauser, K. (2007). Introduction to Geomicrobiology, Blackwell Publishing, Oxford. PMid:17332403
  • Malki, M., González-Toril, E., Sanz, J.L., Gómez, F., Rodríguez, N., Amils, R. (2006). Importance of the iron cycle in biohydrometallurgy. Hydrometallurgy 83 (1–4), 223–228.
  • Malki, M., De Lacey, A.L., Rodríguez, N., Amils, R., Fernandez, V.M. (2008). Preferential Use of an Anode as an Electron Acceptor by an Acidophilic Bacterium in the Presence of Oxygen. Appl. Environ. Microb. 74 (14), 4472–4476. PMid:18487393 PMCid:PMC2493157
  • Malki, M., Casado, S., López, M.F., Caillard, R., Palomares, F., Martín-Gago, J., Vaz-Domínguez, C., Cuesta, A., Amils, R., Fernández, V., Velez, M., De Lacey, A., Olea, D. (2013). Physicochemical Characterization of Acidiphilium Sp. Biofilms. Chem. Phys. Chem. 14 (6), 1237–1244. PMid:23456979
  • McLean, R.J., Beauchemin, D., Beveridge, T.J. (1992). Influence of oxidation state on iron binding by Bacillus Licheniformis capsule. Appl. Environ. Microb. 58 (1), 405–408. PMid:1539987 PMCid:PMC195223
  • Moppert, X., Costaouec, T.L., Raguenes, G., Courtois, A., Simon-Colin, C., Crassous, P., Costa, B., Guezennec, J. (2009). Investigations into the uptake of copper, iron and selenium by a highly sulphated bacterial exopolysaccharide isolated from microbial mats. J. Ind. Microbiol. Biot. 36 (4), 599–604. PMid:19198908
  • Morel, F.M.M., Hering, J.G. (1993). Principles and Applications of Aquatic Chemistry, John Wiley & Sons, New York.
  • Omoike, A., Chorover, J. (2004). Spectroscopic Study of Extracellular Polymeric Substances from Bacillus Subtilis: Aqueous Chemistry and Adsorption Effects. Biomacromolecules 5 (4), 1219–1230. PMid:15244434
  • Sand, W., Gehrke, T. (2006). Extracellular polymeric substances mediate bioleaching/biocorrosion via interfacial processes involving iron(III) ions and acidophilic bacteria. Res. Microbiol. 157 (1), 49–56. PMid:16431087
  • Schmitt, J., Flemming, H. (1998). Ftir-Spectroscopy in microbial and material analysis. Int. Biodeter. Biodegr. 41 (1), 1–11.
  • Tapia, J.M., Mu-oz, J.A., González, F., Blázquez, M.L., Malki, M., Ballester, A. (2009). Extraction of extracellular polymeric substances from the acidophilic bacterium Acidiphilium 3.2sup(5). Water Sci. Technol. 59 (10), 1959–1967. PMid:19474490
  • Tapia, J.M., Mu-oz, J.A., González, F., Blázquez, M.L., Ballester, A. (2011). Mechanism of adsorption of ferric iron by extracellular polymeric substances (Eps) from a bacterium Acidiphilium Sp. Water Sci. Technol. 64 (8), 1716–1722.
  • Tapia, J.M., Mu-oz, J.A., González, F., Blázquez, M.L., Ballester, A. (2013). Sorption of Ferrous and Ferric Iron by Extracellular Polymeric Substances (Eps) from Acidophilic Bacteria. Prep. Biochem. Biotech. 43 (8), 815–827. PMid:23876140
  • Wingender, J., Neu, T.R., Flemming, H.C. (1999). Microbial Extracellular Polymeric Substances. What Are Bacterial Extracellular Polymeric Substances?, Chapter 1, Springer-Verlag, Berlin, pp. 1–19.