Functional activity of recombinant forms of antimüllerian hormone from European sea bass (Dicentrarchus labrax)

  1. C. Zapater 1
  2. A. Rocha 1
  3. G. Molés 1
  4. S. Ibáñez 1
  5. A. Gómez 1
  1. 1 Department of Fish Physiology and Biotechnology, Institute of Aquaculture Torrela Sal, CSIC, Castellon, Spain.
Proceedings:
Advances in Comparative Endocrinology. XII Conference of the Iberian Association for Comparative Endocrinology (AIEC)
  1. Pedro M. Guerreiro (ed. lit.)
  2. João C. R. Cardoso (ed. lit.)

Publisher: Universidade do Algarve / Associação Ibérica de Endocrinologia Comparativa (AIEC)

ISBN: 978-989-9023-31-4

Year of publication: 2021

Pages: 43-46

Type: Conference paper

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Abstract

Anti-Müllerian hormone (AMH) is a member of the transforming growthfactor-β superfamily that classically has been correlated to the regressionof Müllerian ducts in higher vertebrates. Despite the absence of Müllerianducts in teleosts, orthologues of mammalian AMH have been describedin some fish species, and a role of this hormone in sex determination andgonad differentiation has been demonstrated. In adult teleost gonads,Amh acts at early stages of germ cell development in both males andfemales, however, the exact mechanism of Amh signaling and itsimplication in gonad development are poorly investigated. We haverecently demonstrated that Amh signals through a transmembrane AMHtype II receptor (Amhr2) with serine-threonine kinase activity, and thatAmh is localized in Sertoli cells surrounding early germ-cell generations,but still lacking knowledge about the function of this growth factor. As toolfor studying the mechanisms of Amh action, we have produced specificrecombinant mature forms of this hormone, and have used them in invitro transactivation experiments to study the intracellular signalingpathways of sea bass Amhr2. In addition, we have performed in vitrotissue cultures of ovaries and testis treated with recombinant Amh tostudy the impact of this hormone in steroidogenesis, and its interactionwith Fsh, both by analyzing steroid production and gene expression.We finally concluded that Amh promotes steroid production in immaturetestis and early vitellogenic ovaries, showing a clear involvement of seabass Amh in gametogenesis.

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