Analysis of phenotypic and molecular diversity of Phaeomoniella chlamydospora isolates in Spain

  1. Tello, M.L. 1
  2. Gramaje, D. 2
  3. Gómez, A. 1
  4. Abad-Campos, P. 2
  5. Armengol, J. 2
  1. 1 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario
    info

    Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario

    Madrid, España

  2. 2 Universidad Politécnica de Valencia
    info

    Universidad Politécnica de Valencia

    Valencia, España

    ROR https://ror.org/01460j859

Journal:
Journal of Plant Pathology

ISSN: 1125-4653

Year of publication: 2010

Volume: 92

Issue: 1

Pages: 195-203

Type: Article

SCOPUS: 2-s2.0-79956196601 GOOGLE SCHOLAR

More publications in: Journal of Plant Pathology

Abstract

Phenotypic and molecular diversity of 57 Phaeomoniella chlamydospora isolates obtained in 2001-2005 from diverse Spanish viticultural areas and scion/rootstock combinations was investigated. Reference isolates of this species were included in the analysis. For phenotypic characterization, mycelial growth rates at 5, 10, 15, 20, 25, 30 and 35oC, sporulation on potato dextrose agar (PDA) and production of the Phoma-like synanamorph on carnation leaf agar (CLA) were studied. Molecular characterization was performed using five Inter-Simple Sequence Repeat (ISSR) and 24 random amplified polymorphic DNA (RAPD) markers. Both phenotypic and molecular data showed strong homogeneity among all Spanish P. chlamydospora isolates, regardless of their geographical origin, year of isolation and scion/rootstock combination.