Modelo de biofilm bacteriano in vitroevaluación en superficies de implantes y comparación de agentes antimicrobianos

Abstract

Biofilms are complex microbial communities developed on solid surfaces exposed to awet environment. Oral biofilms are considered the etiologic agents of the mostimportant oral diseases. In the oral cavity, different biofilms may be encounteredattached to different types of surfaces including teeth, prosthetic devices and dentalimplants. The formation and maturation of biofilms over dental implant surfaces mayhave pathogenic implications in the development of peri-implant diseases, such asperi-implant mucositis or peri-implantitis.In spite of the similarities between the biofilms on tooth or implant surfaces, somebiofilm features may be attributed to the implant´s specific micro- and macroscopicsurface characteristics. Previous in vitro and in vivo studies have reported that somesurface characteristics like roughness, surface free energy, wettability and degree ofsterilization may affect biofilm formation and the bacterial three- dimensionaldistribution, although there is controversy on the existence of the specific mechanism.Similarly, if the surface can affect the formation and development of the biofilm, theeffectiveness of different antiseptics over that biofilm can be also affected. With thepurpose of studying the implant surface bacterial interactions, several in vitro biofilmmodels have been tested and validated...