Aplicación de las técnicas "ómicas" en la búsqueda de genes implicados en patologías alérgicas asociadas a la infección por "Anisakis simplex" s.l.

Abstract

The allergic reactions associated with the A. simplex s.l. infections cause constant clinical demand in our country. During the last years, both diagnosis and knowledge of the anisakiasis different clinical entities have considerably increased, although they are several aspects associated to the peculiar nature of the disease still unresolved. The human infection by A. simplex s.l. is an accidental parasitosis that triggers allergic reactions as mechanisms for the nematode larvae expulsion. There are several clinical phenotypes associated to Anisakis spp. infection that are largely accepted; for example, gastro-allergic anisakiasis (GAA) or A. simplex s.l. sensitization associated chronic urticaria (CU+). In contrast, occupational asthma and/or contact dermatitis cases by Anisakis spp. sensitization were reported, even though the relationship between the disorders and the nematode has not been explained so far. In addition, there is a big antigenic community, including allergens, non-characterized and shared by both nematodes and arthropods, which are responsible of the frequent cross-reactions observed between the two groups of organisms.Based on this background, the main goal of the present work was the search, identification and molecular characterization of new allergens (theoretical and experimentally demonstrated) from A. simplex s.l. larvae 3, using “omics” approaches...