Fases previa y postcongelación del semen de verraco en pajillas de 5 ml y capacidad de fecundación de los espermatozoides

Resumo

The objective of this study was to determine the effect of the previous and later phases to the freezing of the boar semen in straws of 5 ml about the fecundation capacity in vitro (IVF) of the sperms. 21 ejaculated of seven different animals were used to, compared fresh semen, treaty (semen with all the necessary components to carry out the freezing, before being subjected to the phase of vapors of liquid nitrogen) and semen frozen in straws of 5 ml. The results were 93.81, 87.23 and 78.47 % of spermatic penetration; 81.57, 76.44 and 69.11 % monospermy; 12.24, 10.79 and 9.36 % polyspermy; 84.76, 84.28 and 47.14 % of motility; 79.76, 73.33 and 44.81 % of normal acrosomes (NAR) for fresh semen, treaty and thawed, respectively. When carrying out the variance analysis and the multiple comparisons test, they were differences statistically significant (p<0.05) among fresh semen, treaty and thawed. Therefore, the decrease in the answer of the sperms to the IVF is already observed from the previous phase to the freezing. However, the results obtained with the IVF monospermyc, are promising for the use of the hog thawed semen in the artificial insemination