Nuevo modelo celular para el estudio de la señalización de insulina en miocardio

Resumen

In the last years, the prevalence of obesity and metabolic diseases like diabetes type 2 and metabolic syndrome, suffered an increase. Underlying effect in people with obesity with or without diabetes type 2, are insulin resistance and cardiovascular disease that are one of the major cause of death. Nevertheless, the action of insulin resistance over cardiac function is unknown. In this work, we studied the insulin signaling and its resistance in mice neonatal cardiomyocytes. We generated a new cellular line of immortalized cardiomyocytes. In these cells we studied the expression of several proteins of insulin signaling pathway like insulin receptor and their major substrates IRS-1 and IRS-2, besides PKB/Akt pathway, ERKs and p70/S6K. We also studied some ß-oxidation enzymes like Acetyl-CoA Carboxilase and AMPK. Finally, we studied glucose uptake with 2-Deoxy-glucose under differents stimuli. The results showed that our new cell line are insulin and free fatty acids sensitive but it presents insulin resistance to glucose uptake which can be due to a low expression of GLUT4. In conclusion, these new cellular model may be useful to study glucose intolerance associated to obesity that appears in diabetes type 2.