Cytotoxic activity of α-humulene and transcaryophyllene from Salvia officinalis in animal and human tumor cells

  1. Hadri, Adil el
  2. Gómez del Río, María Angeles
  3. Sanz, Jesús
  4. González Coloma, Ana Azucena
  5. Idaomar, Mohammed
  6. Ribas Ozonas, Bartolomé
  7. Benedí González, Juana
  8. Sánchez Reus, María Isabel
Revista:
Anales de la Real Academia Nacional de Farmacia

ISSN: 1697-4298 0034-0618

Año de publicación: 2010

Número: 3

Páginas: 343-356

Tipo: Artículo

Otras publicaciones en: Anales de la Real Academia Nacional de Farmacia

Resumen

Background: The purpose of the present work is two-fold: the fractionation of Salvia officinalis essential oil and the cytotoxic study of this oil with its fractions �in vitro� tumor cell lines. Materials and Methods: S. officinalis essential oil was obtained by hydrodistillation and fractionated with column chromatography; the essential oil and its fractions were analyzed by gas chromatography (GC) coupled to mass spectrometry (MS). The cytotoxic activity was evaluated in cellular lines of breast cancer MCF-7, colon cancer HCT-116, and murine macrophage RAW264.7 cell lines by the MTT assay. Results: the sub-subfraction F1.1.1 of S. officinalis essential oil containing a-humulene present highest activity on RAW264.7 and HCT-116 with IC50 values of 41.9 and 77.3 µg/ml, respectively. The sub-subfraction F1.2.1 of S. officinalis essential oil with trans-caryophyllene showed less activity on RAW246.7 and HCT-116 with IC50 values of 90.5 and 145.8 µg/ml. Conclusion: This paper suggests that the a-humulene and trans-caryophyllene extracted from S.officinalis essential oil inhibit tumor cell growth.