Efectos de la infección in vitro de células monocítico-macrofágicas por Chlamydia y su relación con los valores de lípidos
- Millán Núñez-Cortés, Jesús
- Recarte García Andrade, Carlos
- López Paredes, Cristina
- Domingo, Félix
- Teigell García, Luis
- Filgueira Rubio, José
- Álvarez Novés, Granada
ISSN: 0214-9168, 1578-1879
Year of publication: 2011
Volume: 23
Issue: 5
Pages: 201-210
Type: Article
More publications in: Clínica e investigación en arteriosclerosis
Abstract
Introduction: The mononuclear-fagocitic cellular type is a key element during the atherogenic process. The monocyte takes a transcendental role in the inflammatory process that is relevant at the onset and subsequent development of atheroma plaque. Otherwise, the cellular infiltration is a key feature to consider during the inflammatory process that we can found related with the atherosclerosis/infection association. According with the infectious explanation of atherosclerosis, microorganism more frequent associated with vascular pathology has been: Citomegalovirus, Helicobacter and Chlamydia. In the present paper we are interested to evaluate the characteristics and cellular behaviour of cultures of cells related with atherogenic process that has been previously infected with Chlamydia. Material and methods: We have analized established human endothelial cells (Hep-2 cells), established mononuclear cells (THP-1 cells) and primary cultures of monocytes from blood health volunteers. Results: All of cells types has been easily infected by Chlamydia. Nevertheless, the effects are different according with the different cells types. When we have compared the effect of normo or hyperlipidemic serum specimens over the cultures of infected mononuclear cells it is possible to show that the effect of infection is amortiguated by the presence of high serum levels of lipids.