La depleción de células CD34+/CD144+ en pacientes de revascularización miocárdica no está mediada por un aumento de la apoptosis inducida por el TGF-beta plasmático
- Ramajo, Marta
- Redondo Blasco, Santiago
- González Rocafort, A.
- Navarro Dorado, Jorge
- Reguillo La Cruz, Fernando
- Carnero, Manuel
- Martínez González, José
- Rodríguez, Enrique
- Tejerina, Mª Teresa
ISSN: 0214-9168, 1578-1879
Année de publication: 2012
Volumen: 24
Número: 1
Pages: 12-20
Type: Article
D'autres publications dans: Clínica e investigación en arteriosclerosis
Résumé
Introduction: Coronary atherosclerotic disease has been linked to endothelial progenitor cell (EPC) depletion and functional impairment. We assessed the number of CD34+/KDR+ and CD34+/CD144+ cells in coronary artery bypass grafting (CABG) patients, compared to valvular patients, and the apoptotic effect of the plasma from these two groups on early outgrowth cells (EOCs) from healthy donors. Methods: CD34+/KDR+ and CD34+/CD144+ cell numbers were assessed by flow cytometry. EOCs were obtained from buffy coats from healthy donors. Apoptosis was measured as DNA fragmentation. In vitro expression of CD34 and CD144 was assessed by Western blot. Results: We found a lower number of CD34+/CD144+ cells in CABG patients compared to valvular, but a higher number compared to healthy controls. Plasma from CABG patients decreased apoptosis in cultured EOCs from healthy donors (day 7); this effect was abrogated by the TGF-beta1 blockers SIS3 and SB-431542. Prior to apoptosis protection, in cultured cells from healthy donors (day 4), plasma from CABG increased CD34 and CD144 expression, contrary to what found in the blood of the patients. Conclusions: CABG patients possess a lower number of CD34+/CD144+ cells than valvular ones. Plasma from CABG patients is antiapoptotic for EOCs from healthy donors and increases the expression of both CD34 and CD144. Suboptimal number of CD34+/CD144+ in vivo may be mediated by a primary bone marrow defect rather than a deleterious effect of plasma.