Tratamiento comparado de las lesiones del manguito rotador mediante el uso de células madre mesenquimales en combinación con membranas de colágeno tipo I
- P. Tornero Esteban
- J. A. Hoyas
- E. Villafuertes
- C. Rodríguez Bobada
- Gustavo Víctor Guinea Tortuero
- Yaiza Lópiz Morales
- Benjamín Fernández Gutiérrez
- Fernando Marco Martínez
ISSN: 1888-6116
Argitalpen urtea: 2012
Alea: 23
Zenbakia: 1
Orrialdeak: 70-75
Mota: Artikulua
Beste argitalpen batzuk: Trauma
Laburpena
Objective: To study, in a rat model, the supraspinatus tendon (SE) rupture with isolated MSCs or combined with collagen membranes Material and method: A chronic rotator cuff tear injury model was developed by unilaterally detaching the SE tendon of aged Sprague-Dawley rats (9 months). One month post-injury, a second surgery was then performed to repair the tears by: 1. single suture (n=30), 2. suture and type I collagen membranes (n=30) and 3. suture and type I collagen membranes with 1x106 allogeneic MSCs; (n=30). Lesion restoration was evaluated at one month (n=30), two months (n=30) and three months (n=30) post-injury by biomechanical, histological and inmunohistological criterias. Results: All experimental conditions were well tolerated and no adverse effects were observed. Implantation of collagen type I membrane with MSCs into surgically created tendon defects improved strength and stiffness of SE tendon three months after treatment compared with the other two repair surgeries. No treatment achieved a histological organized pattern. Conclusion: The therapeutic use of allogenic MSCs in collagen type I membrane in the rotator cuff tears seems to be the most promising treatment in our experimental model.