Influence of phenological stage on the antioxidant activity of Thymus zygis s. l. essential oil

  1. Blanco Salas, José
  2. Ruiz Téllez, Trinidad
  3. Vázquez Pardo, Francisco María
  4. Cases Capdevila, María Angeles
  5. Pérez Alonso, María José
  6. Gervasini Rodríguez, C.
Revista:
Spanish journal of agricultural research

ISSN: 1695-971X,  2171-9292

Año de publicación: 2012

Volumen: 10

Número: 2

Páginas: 461-465

Tipo: Artículo

DOI: 10.5424/SJAR/2012102-640-11 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Spanish journal of agricultural research

Resumen

When a species has proven antioxidant capacity and possible commercial application it is important to know whether or not this activity varies with the phenological stage of the raw material commodity. The present work aims to assess this topic for the case of Thymus zygis s.l. We analyzed the yield and the chemical composition (gas chromatography?mass spectrometry) of its essential oil in five wild populations from the Southwest of Iberian Peninsula at flowering and fruiting stage. We measured their TEAC (Trolox equivalent antioxidant activity) values. The main components were p-cymene (flowering: 33.6-16.2%; fruiting: 43.2-30.3%), ?-terpinene (flowering: 13.5-5.78%; fruiting: 4.67-1.96%) and thymol (flowering: 46.45-22.2%; fruiting: 54.24-13.9%); the latest, especially high (flowering: 46.45%; fruiting: 54.24%) in one of the populations, identified as Thymus zygis ssp. gracilis (Boiss.) R. Morales, which showed the highest antioxidant activity as well. In the set of samples TEAC oscillated between 16.12-30.52 mmol Trolox L-1 (flowering) and 10.96-40.24 mmol Trolox L-1 (fruiting). No significant differences between antioxidant activity of both stages were found, but yields were significantly higher at flowering time. These levels of antioxidant activity are close to those achieved as highly antioxidant food products, as coffee or wine. This species can be a good raw material for agribusiness use, specially the population of Thymus zygis ssp. gracilis.

Referencias bibliográficas

  • Antolovich M, Prenzler PD, Patsalides E, Mcdonald S, Robards K, 2002. Methods for testing antioxidant activity. Analyst 127: 183-198. http://dx.doi.org/10.1039/b009171p PMid:11827390
  • Arnao MB, Cano A, Acosta M, 2000. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73: 239-244. http://dx.doi.org/10.1016/S0308-8146(00)00324-1
  • Blanco J, 2005. Contribución al conocimiento de los recursos fitogenéticos de Extremadura: el caso de los tomillos. Doctoral Thesis. Univ. Extremadura. [In Spanish].
  • Blanco J, Ruiz T, Pérez-Alonso MJ, Vázquez FM, Cases MA, Gervasini C, 2010. Chemical composition and antioxidant activity of the essential oil of Thymbra capitata (L.) Cav. in Spain. Acta Bot Gallica 157: 55-63.
  • Cano A, Acosta M, Arnao MB, 2000. A method to measure antioxidant activity in organic media: Application to lipophilic vitamins. Redox Report 5: 365-370. http://dx.doi.org/10.1179/135100000101535933 PMid:11140747
  • Council of Europe, 1996. European Pharmacopoeia. 3rd. Strasbourg, France, 1799 pp.
  • Dorman HJD, Figueiredo AC, Barroso JG, Deans SG, 2000a. In vitro evaluation of antioxidant activity of essential oils and their components. Flav Fragr J 15: 12-16. http://dx.doi.org/10.1002/(SICI)1099-1026(200001/02)15:1<12::AID-FFJ858>3.0.CO;2-V
  • Dorman HJD, Surai P, Deans SG, 2000b. In vitro antioxidant activity of a number of plant essential oils and phytoconstituents. J Essent Oil Res 12: 241-248.
  • Figueiredo AC, Barroso J G, Pedro LG, Salgueiro L, Miguel MG, Faleiro ML, 2008. Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. Curr Pharm Design 14: 3120-3140. http://dx.doi.org/10.2174/138161208786404218 PMid:19075695
  • Jiménez J, Navarro MC, Montilla MP, Martín A, Martínez A, 1993. Thymus zygis oil: its effects on CC14-induced hepatotoxicity and free radical scavenger activity. J Essent Oil Res 5: 153-158.
  • Jordán MJ, Martínez RM, Martínez C, Moñino I, Sotomayor JA, 2009. Polyphenolic extract and essential oil quality of Thymus zygis ssp. gracilis shrubs cultivated under different watering levels. Ind Crops Prod 29: 145-153. http://dx.doi.org/10.1016/j.indcrop.2008.04.021
  • Morales R, 2010. Thymus L. In: Flora Ibérica XII (Morales R, Quintanar A, Cabezas F, Pujadas AJ, Cirujano S eds). CSIC, Madrid. pp: 349-409. [In Spanish].
  • Pellegrini N, Serafini M, Colombi B, Del Río D, Salvatore S, Bianchi M, Brighenti F, 2003. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three diferent in vitro assays. J Nutr 133: 2812-1819. PMid:12949370
  • Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R, 2005. Comparative evaluation of essential oils of different origin as functional antioxidant, antiradicals and antimicrobials in foods. Food Chem 91: 621-632. http://dx.doi.org/10.1016/j.foodchem.2004.06.031
  • Sotomayor JA, 1998. Estudio sobre plantas aromáticas de los géneros Salvia y Thymus, espontáneas en el Sureste Ibérico, para su establecimiento como cultivo. Doctoral Thesis. Univ Murcia, Murcia, Spain. [In Spanish].
  • Vazquez FM (coord), 2008. Lamiaceae. In: Plantas Medicinales en la Comunidad de Extremadura. Diputación de Badajoz, Badajoz, Spain. pp: 279-319. [In Spanish].
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