Análisis de los métodos de filtraciónXI Premio Científico Anual Prof. DR. D. Pedro Ruiz de Temiño (Ex aequo)

Resum

Several systems have been used to assess the sealing capacity of different obturation materials in the root canal. Given the diversity of materials and techniques the results are hard to compare. The objective of the study is to determine whether the clearing process is capable of eliminating the dye used to analyse filtration, and whether the results obtained by means of clearing and longitudinal sectioning are comparable. Material and method: we prepared 125 single-canal roots from extracted teeth and divided them into 5 groups of 20 samples; each group was immersed in a different dye . The dyes we used were rhodamine, eosin, hematoxylin, Indian ink and methylene blue. Half the group underwent clearing and the other half were longitudinally sectioned. We took photographs of the samples and studied them with the help of digital image analysing software. The remaining roots were used as positive and negative controls. Results: With the clearing method we found significant differences of up to 95% between methylene blue and the other dyes, and Indian ink and the other dyes. There were no significant differences between eosin, rhodamine and hematoxylin. Using the sectioning method, we observed significant differences of up to 95% between methylene blue and eosin, hematoxylin and rhodamine. There were no significant differences between the other dyes. There were significant differences in the results obtained by the clearing method and longitudinal sectioning, with all of the dyes apart from Indian ink. The results of our study demonstrate the need for a unified technique and unified material to compare results successfully. Conclusions: depending on the dye, the results of the clearing process differ. The results of clearing and longitudinal sectioning are incomparable, except in the case of Indian ink.