Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

  1. Rocío Velázquez Cayón 1
  2. Gabriel Castillo Dalí 1
  3. José Ramón Corcuera Flores 2
  4. María Angeles Serrera Figallo 2
  5. Raquel Castillo de Oyagüe 3
  6. Maribel González Martín
  7. José Luis Gutiérrez Pérez 4
  8. Daniel Torres Lagares 5
  1. 1 DDS, PhD, Master’s Degree in Oral Surgery. School of Dentistry. University of Seville
  2. 2 DDS, PhD, Associate Professor, Master’s in Integrated Dentistry and Patients with Special Diseases. School of Dentistry. University of Seville
  3. 3 DDS, PhD, Associate Professor, PhD in Stomatological Prosthesis. School of Dentistry of the Complutense University of Madrid
  4. 4 DMD, PhD, Affiliated Professor of the Master’s Degree in Oral Surgery. School of Dentistry. University of Seville
  5. 5 DDS, PhD, Professor of the Master’s Degree in Oral Surgery. School of Dentistry. University of Seville
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Revista:
Medicina oral, patología oral y cirugía bucal. Ed. inglesa

ISSN: 1698-6946

Año de publicación: 2017

Volumen: 22

Número: 5

Páginas: 19

Tipo: Artículo

DOI: 10.4317/MEDORAL.22071 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Medicina oral, patología oral y cirugía bucal. Ed. inglesa

Resumen

The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it.

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Fuente de los datos: Dialnet