Detecciónes de la expresión de citoquinas mediante RT-PCR en tiempo real en ganado ovino

  1. Ana Cristina Pérez de Diego Camacho 1
  2. Almudena Sánchez Matamoros 1
  3. José Manuel Sánchez-Vizcaíno Rodríguez
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Journal:
Revista complutense de ciencias veterinarias

ISSN: 1988-2688

Year of publication: 2009

Volume: 3

Issue: 2

Pages: 129-138

Type: Article

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Abstract

Cytokines are protein molecules that cells from the immune system secrete in response to immune stimulation. There are different techniques to study their production and their action on individuals. This study consists of the development of a real time RT-PCR method for the detection of the expression of mRNA coding for six cytokines (TNF-α, IFNγ, IL-4, IL2, IL- 10, IL-12). The preliminary results were obtained from the study of lymphocites stimulated with Con A and LPS, showing the detection of ARNm, IL-4, IL-2, IFNγ and TNF-α, IL-12, IL-10, respectively. Its subsequent application in sheep that were vaccinated against Chlamydophila abortus, allowed the detection of mRNA expression for the six cytokines.These results show that it is a swift, sensitive and reliable technique to detect mRNA of cytokines.

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