Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

  1. Vicente Rubiano, M.
  2. Kukielka Zunzunegui, Deborah
  3. Heras, A. I. de las
  4. Sánchez-Vizcaíno Rodríguez, José Manuel
Revista:
Spanish journal of agricultural research

ISSN: 1695-971X,  2171-9292

Año de publicación: 2013

Volumen: 11

Número: 3

Páginas: 708-713

Tipo: Artículo

DOI: 10.5424/SJAR/2013113-3531 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Spanish journal of agricultural research

Resumen

This study aimed to assess the possible relationship between the presence of Israeli acute paralysis virus (IAPV) of honeybees and disease symptoms development at the colony level, to describe the IAPV load in field colonies and to illustrate phylogenetic relationships between IAPV isolates in Andalusia (Spain). Presence and load of IAPV was studied in 96 colonies from all provinces in Andalusia. Epidemiological surveys were performed in all the colonies to assess their sanitary status. IAPV was found in 13.5% of the sampled colonies, and no association was observed between the presence of IAPV and disease symptoms at the colony level. An average IAPV load was established in 4.9�105 genome equivalent copies per bee. Phylogenetic analysis revealed that Andalusian isolates belong to a different lineage to a previously described isolate found in Valencia (2010). The results of this study will help us understand the epidemiology and effect of IAPV on Spanish colonies.

Referencias bibliográficas

  • Antúnez K, Anido M, Garrido-Bailón E, Botías C, Zunino P, Martínez-Salvador A, Martín-Hernández R, Higes M, 2012. Low prevalence of honeybee viruses in Spain during 2006 and 2007. Res Vet Sci 93(3): 1441-1445. http://dx.doi.org/10.1016/j.rvsc.2012.03.006 PMid:22513127
  • Aronstein KA, Murray KD, 2010. Chalkbrood disease in honey bees. J Invertebr Pathol 103 (Suppl 1): S20-29. http://dx.doi.org/10.1016/j.jip.2009.06.018 PMid:19909969
  • Blanchard P, Schurr F, Celle O, Cougoule N, Drajnudel P, Thiery R, Faucon JP, Ribière M, 2008. First detection of Israeli acute paralysis virus (IAPV) in France, a dicistrovirus affecting honeybees (Apis mellifera). J Invertebr Pathol 99: 348-350. http://dx.doi.org/10.1016/j.jip.2008.07.006 PMid:18703069
  • Cox-Foster DL, Conlan S, Holmes EC, Palacios G, Evans JD, Moran NA, Quan PL, Briese T, Hornig M, Geiser DM et al., 2007. A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318: 283-287. http://dx.doi.org/10.1126/science.1146498 PMid:17823314
  • De Blas N, Ortega C, Frankena K, Noordhuizen J, Thrusfield M, 2000. WinEpiscope version 2.0. University of Edinburgh. PMCid:PMC27650
  • De Miranda JR, Drebot M, Tyler S, Shen M, Cameron CE, Stoltz DB, Camazine SM, 2004. Complete nucleotide sequence of Kashmir bee virus and comparison with acute bee paralysis virus. J Gen Virol 85: 2263-2270. http://dx.doi.org/10.1099/vir.0.79990-0 PMid:15269367
  • De Miranda JR, Cordoni G, Budge G, 2010. The acute bee paralysis virus-Kashmir bee virus-Israeli acute paralysis virus complex. J Invertebr Pathol 103 (Suppl 1): S30-S47. http://dx.doi.org/10.1016/j.jip.2009.06.014 PMid:19909972
  • Di Prisco G, Pennacchio F, Caprio E, Boncristiani HF, Evans JD, Chen Y, 2011. Varroa destructor is an effective vector of Israeli acute paralysis virus in the honeybee, Apis mellifera. J Gen Virol 92: 151-155. http://dx.doi.org/10.1099/vir.0.023853-0 PMid:20926637
  • Garrido-Bailón E, Martín-Hernández R, Bernal J, Bernal JL, Martínez-Salvador A, Barrios L, Meana A, Higes M, 2010. Short communication. The detection of Israeli Acute Paralysis virus (IAPV), fipronil and imidacloprid in professional apiaries are not related with massive honey bee colony loss in Spain. Span J Agric Res 8: 658-661.
  • Gauthier L, Tentcheva D, Tournaire M, Dainat B, Cousserans F, Colin ME, Bergoin M, 2007. Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique. Apidologie 38: 426-435. http://dx.doi.org/10.1051/apido:2007026
  • Glinski Z, Buczek K, 2003. Response of the Apoidea to fungal infections. Apiacta 38: 183-189.
  • Govan VA, Leat N, Allsopp M, Davison S, 2000. Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses. Virology 277: 457-463. http://dx.doi.org/10.1006/viro.2000.0616 PMid:11080493
  • Kukielka D, Sánchez-Vizcaíno JM, 2010. Short communication. First detection of Israeli Acute Paralysis Virus (IAPV) in Spanish honeybees. Span J Agric Res 8: 308-311.
  • MAGRAMA, 2012. El sector de la miel en cifras. Principales indicadores económicos de 2011. Spanish Ministry of Agriculture, Food and Environment. Available in http://www.magrama.es/ [March 2012].
  • Maori E, Lavi S, Mozes-Koch R, Gantman Y, Peretz Y, Edelbaum O, Tanne E, Sela I, 2007. Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination. J Gen Virol 88: 3428-3438. http://dx.doi.org/10.1099/vir.0.83284-0 PMid:18024913
  • Maori E, Paldi N, Shafir S, Kalev H, Tsur E, Glick E, Sela I, 2009. IAPV, a bee-affecting virus associated with colony collapse disorder can be silenced by dsRNA ingestion. Insect Mol Biol 18(1): 55–60. http://dx.doi.org/10.1111/j.1365-2583.2009.00847.x PMid:19196347
  • Martin SJ, Highfield AC, Brettel L, Villalobos EM, Budge GE, Powell M, Nikaido S, Schroeder DC, 2012. Global honey bee viral landscape altered by a parasitic mite. Science 336(6086): 1304-1306. http://dx.doi.org/10.1126/science.1220941 PMid:22679096
  • Palacios G, Hui J, Quan PL, Kalkstein A, Honkavuori KS, Bussetti AV, Conlan S, Evans J, Chen YP, vanEngelsdorp D, et al., 2008. Genetic analysis of Israel acute paralysis virus: distinct clusters are circulating in the United States. J Virol 82: 6209-6217. http://dx.doi.org/10.1128/JVI.00251-08 PMid:18434396 PMCid:PMC2447087
  • Pohorecka K, Zdanska D, Bober A, Skubida M, 2011. First detection of Israeli acute paralysis virus (IAPV) in Poland and phylogenetic analysis of the isolates. J Apicult Sci 55(2): 149-159.
  • Reynaldi FJ, Sguazza GH, Tizzano MA, Fuentealba N, Galosi CM, Pecoraro MR, 2011. First report of Israeli acute paralysis virus in asymptomatic hives of Argentina. Rev Argent Microbiol 43: 84-86. PMid:21731968
  • Ribière M, Ball B, Aubert MFA, 2008. Natural history and geographical distribution of honey bee viruses. In: Virology and the honey bee (Aubert M, Ball B, Fries I, Moritz R, Milani N, Bernardinelli I, eds.). European Commission, Brussels, pp: 15-84.
  • Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: molecular evolutionary genetics analysis software version 4.0. Mol Biol Evol 24: 1596-1599. http://dx.doi.org/10.1093/molbev/msm092 PMid:17488738
  • Xun Y, XueJiao L, JingHua C, Yi Z, RiChou H, 2009. First detection of Israel acute paralysis virus (IAPV) in Apis mellifera (Hymenoptera: Apidae) in China. Sociobiology 54: 95-100.
  • Yang Y, Cox-Foster DL, 2005. Impact of an ectoparasite on the immunity and pathology of an invertebrate: Evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci 102(21): 7470-7475. http://dx.doi.org/10.1073/pnas.0501860102 PMid:15897457 PMCid:PMC1140434
Fuente de los datos: Dialnet