Protein adsorption and macrophage polarization as biocompatibility markers for biomaterial design

Abstract

The standard protocol before proceeding to clinical trials of a biomaterial is to follow a battery of determined in vitro following in vivo experimentation. The lack of correlation between in vitro and in vivo experimentation is a problematic when addressing material biocompatibility. he characterisation of the layer of proteins formed post-implantation using mass spectrometry, comes as a tool to assess patterns of clusters of proteins related with biocompatibility problems. These clusters of proteins can be responsible for macrophage activation at the moment of implantation. he study of inflammatory markers expressed by each phenotype of macrophages when cultured onto biomaterials can be an effective tool to approach the inflammatory response to a determined material. his thesis addresses the possibility of adopting these two methodologies as new potential tools to approach in vitro experimentationon this field. Proteomic, in vitro and in vivo characterisation was made and correlated regarding biocompatibility characterization.