Caracterización y selección in vivo de espermatozoides de toro mediante lectinas fluorescentesCharacterization and selection of living bull spermatozoa using fluorescent lectins

  1. López Rodas, V 1
  2. M. Jiménez Cabras 2
  3. M.J. Avila Cantariño 1
  4. E. Costas Costas
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

  2. 2 CENSIRA
Journal:
Archivos de zootecnia

ISSN: 0004-0592

Year of publication: 1993

Volume: 42

Issue: 158

Pages: 253-262

Type: Article

DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Archivos de zootecnia

Abstract

Spermatozoa from frison bulls were analysed in vivo by FITC conjugate lectins. The binding activity of lectins was detected by direct observation of the live spermatozoa in a epif uorescence microscope. The spermatozoa head appeared as a zone with great richness in lectin receptors. Also the tail surface showed many glucide rests, just contrarily to conecting piece. Lectins appear as an interesting tool to differenciate among spermatozoa, and so, static as well as slow spermatozoa were bind by different lectins, wich were not able to bind rapide spermatozoa. Due to lectins were also able to agglutinate spermatozoa, the use of Pisum sativa lectin allowed us to separate the rapide spermatozoa fron slow or statics ones. Thus, lectins appear as an interesting tool to characterize and differenciate among spermatozoa.

Data source: Dialnet