Utilidad del test aptima en el diagnóstico de las lesiones precancerígenas del cérvixcomparación de Aptima con otros test diagnósticos

  1. M.P. Cano
  2. A. Sáez
  3. I. Romero
  4. José Manuel Bajo Arenas
  5. J. Valero de Bernabé
Journal:
Toko-ginecología práctica

ISSN: 0040-8867

Year of publication: 2017

Issue: 753

Pages: 127-133

Type: Article

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Abstract

Objectives: The aim of this study is to evaluate the utility of the APTIMA assay, which identifies E6/E7 mRNA of 14 types of high-risk human papillomavirus (HR-HPV), for the diagnosis of grade 2 or higher cervical intraepithelial neoplasia (CIN), and to compare the sensitivity of this test with other diagnostic tests. Methods: A total of 203 patients, referred for a colposcopy due to an abnormal cytology or a positive test for HR-HPV, were evaluated with the APTIMA, Hybrid Capture (HC2), cobas 4800, Linear Array and p16INK4a immunohistochemical staining assays. Results: Sensitivity was calculated for all the tests, using CIN2+ and CIN3+ as endpoints, with sensitivity values above 90% observed for all the diagnostic tests. The sensitivity for APTIMA was 92.80% and 92.94% for CIN2+ and CIN3+ respectively (p<0.0001), for HC2 96.80% and 95.29% (p<0.0001), for cobas 4800 96.00% and 95.29% (p=0.011), for Linear Array 98.40% and 97.65% (p=0.007) and for p16INK4a immunohistochemical staining 95.90% and 96.39% (p<0.0001). The APTIMA assay and the p16INK4a immunohistochemical staining had the best specificity values, 35.90% and 47.94% respectively (p<0.0001). Conclusion: APTIMA has a sensitivity similar to that of other diagnostic tests for the detection of CIN2+, which is why it could be a useful test for cervical cancer

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