Procedimiento de validación de un método para cuantificar cromo en suero por espectroscopía de absorción atómica con atomización electrotérmica
- Irene Palazón Bru
- Lorena López Lozano
- Antonio Palazón Bru
- Manuel Arroyo Fernández
- Monserrat González Estecha
ISSN: 1888-4008
Ano de publicación: 2015
Volume: 8
Número: 1
Páxinas: 52-57
Tipo: Artigo
Outras publicacións en: Revista del laboratorio clínico
Obxectivos de Desenvolvemento Sustentable
Resumo
The interest of measuring chromium in serum is due to the problem with metal-on-metal bearings. They release this metal into tissues and the blood circulation producing harmful effects on health. The aim of this study is to validate a method for chromium determination in serum samples by electrothermal atomization atomic absorption spectrometry technique with longitudinal Zeeman-effect background correction. The features of the method were proved. The detection and quantification limits were 0.074 y 0.247 μg/L, respectively. The characteristic mass was 7.1 pg/0.0044 absorbance units. The calibration curve is linear between 0 and 10 μg/L. The slope of the standard addition curve is included within the confidence interval of the calibration curve using aqueous standards, so that, there is not matrix effect. The precision and accuracy were tested using reference material Seronorm Trace Elements Serum. The mean recovery was 99.32%. The proposed method proves to be sensitive, robust, accurate and precise for biomonitoring the concentration of chromium in serum samples as an indicator of health risk.