Procedimiento de validación de un método para cuantificar cobalto en suero por espectroscopia de absorción atómica con atomización electrotérmica
- Lorena López Lozano
- Irene Palazón Bru
- Antonio Palazón Bru
- Manuel Arroyo Fernández
- Monserrat González Estecha
ISSN: 1888-4008
Argitalpen urtea: 2015
Alea: 8
Zenbakia: 1
Orrialdeak: 46-51
Mota: Artikulua
Beste argitalpen batzuk: Revista del laboratorio clínico
Laburpena
The interest of measuring cobalt in serum is due to the problem with metal-on-metal bearings. They release this metal into tissues and the blood circulation producing harmful effects on health. The aim of this study is to validate a method for cobalt determination in serum samples by electrothermal atomization atomic absorption spectrometry technique (ETAAS) with longitudinal Zeeman-effect background correction. The detection and quantification limits were 0.31 y 1.04 μg/L, respectively. The characteristic mass was 17.8 pg/0.0044 absorbance units. The calibration curve is linear between 0 and 50 μg/L. The slope of the standard addition curve is included within the confidence interval of the calibration curve using aqueous standards, so that, there is not matrix effect. The precision and accuracy were tested using reference material Seronorm Trace Elements Serum. The mean recovery was 98.97% The proposed method proves to be sensitive, robust, and precise for biomonitoring the concentration of cobalt in serum samples as an indicator of health risk