Uso de un circuito genético de retroalimentación positiva para el estudio de la señalización de la ruta de integridad celular de Saccharomyces cerevisiae y la identificación de nuevos antifúngicos

Abstract

Signalling pathways mediated by mitogen-activated protein kinases (MAPKs) are essential for cell survival due to their ability to detect changes in the extracellular environment and adaptively respond to them. MAPK pathways are widely distributed among eukaryotic cells and are composed of a three-tiered-protein kinase module, comprising a MAPKKK, a MAPKK, and a MAPK, which are activated by sequential phosphorylation. In general, the stimulus is detected by sensors on the cell surface. They transduce the signal to downstream components of the route, usually a GTPase and a protein kinase, which in turn activates the MAPK module. Lastly, the active MAPK phosphorylates its effectors, mostly transcription factors, which elicit the adaptive response. The high conservation of MAPK pathways among eukaryotes makes the budding yeast Saccharomyces cerevisiae an excellent model for their study. This microorganism presents five MAPK pathways, including the Cell Wall Integrity (CWI) pathway and the High Osmolarity Glycerol (HOG) pathway...