Estudio piloto para la reconstrucción del ligamento cruzado anterior (LCA) con cultivos de fibroblastos autólogos sobre membranas de colágeno I/III
- J. M. López-Alcorocho Sánchez
- Pedro Guillén García
- E. Rodríguez Íñigo
- Isabel Guillén Vicente
- R. Caballero Santos
- M. Guillén Vicente
- F. García Gómez
- Pedro Cuevas Sánchez
ISSN: 1888-6116
Year of publication: 2013
Volume: 24
Issue: 1
Pages: 33-38
Type: Article
More publications in: Trauma
Abstract
Objective: To study the possibility on the use of the in vitro cultured on I/III collagen membranes for the treatment of the broken anterior cruciate ligament (ACL). Patients and methodology: Prospective study performed with 100 ACL biopsies from patients with broken ACL, in which primary cultures of fibroblasts were established. When a number of 20-30 million was reached, they were transferred to I/III collagen membranes, where the architecture of the cells was examined by hematoxilin-eosin stained and the expression of the collagen type I (Col-I), collagen type III (Col-III), tenascin-C and Sox-9 genes by real time PCR. Results: A negative correlation between the age of the patients and the growth rate of the fibroblasts after 30 days of setting-up the culture (p=0.003) was observed. The histology revealed that the cells were disposed within the membrane fibers net, where the cells expressed the characteristic proteins of the ligament: Col-I, Col-III, Sox-9 and tenascin-C. Conclusion: It is possible to establish the primary culture of the ACL-derived fibroblasts, both in vitro and onto collagen I/III porcine membranes without losing their primary features of the ligament cells.