Dynamics of CaCdc10, a septin of Candida albicans, in living cells and during infection
- Alberto González Novo 1
- Javier Jiménez Jiménez 1
- M. Jesús García 1
- Inmaculada Ríos Serrano 2
- Jesús Pla Hontoria 2
- Antonio Jiménez 3
- Miguel Sánchez Pérez 1
- 1 Institute for Biochemical Microbiology, University of Salamanca-CSIC, Spain
- 2 Microbiology Department, Microbiology II, Faculty of Pharmacy, Complutense University of Madrid, Spain
- 3 Medicine Department, Faculty of Medicine, University of Salamanca, Spain
ISSN: 1618-1905
Año de publicación: 2004
Volumen: 7
Número: 2
Páginas: 105-112
Tipo: Artículo
Otras publicaciones en: International microbiology: official journal of the Spanish Society for Microbiology
Resumen
The morphogenetic program in the pathogenic fungus Candida albicans , including the dimorphic transition, is an interesting field of study, not only because it is absent in the commonly used model yeast Saccharomyces cerevisiae, but because of the close relationship between hyphal development and virulence of C. albicans. We studied one of the most important aspects of fungal morphogenesis-the septin ring–in C. albicans. By using a fusion construct to green fluorescent protein (GFP), the subcellular localization and dynamics of C. albicans Cdc10 in the different morphologies that this fungus is able to adopt was identified. The localization features reached were contrasted and compared with the results obtained from Candida cells directly extracted from an animal infection model under environmental conditions as similar as possible to the physiological conditions encountered by C. albicans during host infection. [Int Microbiol 2004; 7(2):105–112]