Detección de polifosfatos de diadenosina en humor acuoso humano
- Castany, M.
- Pintor, J.
- Jordi, I.
- Carceller, A.
- Català, J.
- Bardavío, J.
- Gasull, X
ISSN: 1133-7737
Argitalpen urtea: 2009
Alea: 17
Zenbakia: 4
Mota: Artikulua
Beste argitalpen batzuk: Annals d'oftalmologia: òrgan de les Societats d'Oftalmologia de Catalunya, Valencia i Balears
Laburpena
Purpose: To study the presence of diadenosine polyphosphates in human aqueous humour. Secondary aim: To evaluate differences between glaucoma and no glaucoma patients. Method: Aqueous humour sample was taken from 20 eyes of 20 patients who were scheduled for phacoemulsification. The extraction of sample was done as the first surgical step: 0.1 to 0.2 ml of aqueous humour was collected at the beginning of the cataract surgery through corneal paracentesis using a 27-gauge cannula on a tuberculin syringe. The samples were immediately cooled at –80 ºC and kept frozen and protected from light. Aqueous humour aliquots were analyzed by High Performance Liquid Chromatography for the presence of Ap4A and Ap5A. Results: 10 patients had glaucoma (9 primary open angle glaucoma and 1 pseudoexfoliative glaucoma). AP4A and AP5A were detected in aqueous humour of all patients. AP5A concentration had no statistically differences between glaucoma and no glaucoma group. AP4A had higher concentrations than AP5A and a statistically difference was detected between the two groups (296.5 nM in the glaucoma group vs 27.5 nM in the control group). Conclusion: For the first time both, AP4A and AP5A have been detected in human aqueous humour. Interestingly, AP4A concentration in glaucoma patients is higher than in the control group. According to results in animal models, AP4A may be part of a homeostatic control of intraocular pressure, although the physiopathological role in humans still has to be elucidated.