Efecto neuroprotector de las células madre mesenquimales adultas en las fases iniciales de una lesión traumática de la médula espinal

  1. Javier Vaquero
  2. C. Bonilla Horcajo
  3. L. Otero
  4. C. Aguayo
  5. Santiago de Oya Otero
  6. Mercedes Zurita

ISSN: 1888-6116

Year of publication: 2008

Volume: 19

Issue: 4

Pages: 199-205

Type: Article

More publications in: Trauma


Aim: To study if the intralesional administration of adult mesenchymal stem cells modifies the number of apoptotic nervous cells in the early phases after severe spinal cord injury (SCI). Material and methods: Twenty adult Wistar rats were subjected to SCI at dorsal level. Two hours after the lesion, in 10 animals a volume of 10 µl of saline into the lesion were administered. Other 10 animalsreceived 106 bone marrow stromal cells obtained. Five animals of each group were sacrificed at the 8 hours after the lesion and the other 5 animals at the 72 hours. The spinal cord was processed in each animal for conventional histological studies and to identify cells in apoptosis using the marker Mab F7-26 (apostain). The number of cells in apoptosis with appearance of neurons or glial cells was quantified in each group, averaged and subjected to statistical comparisons. Results: In the control group the number of nervous cells expressing apostain was 138 ± 16, at the 8 hours and 82 ± 13 at the 72 hours after lesion. In the treated group the number of nervous cells expressing apostain was 76 ± 11, at the 8 hours, and 57 ± 7, at the 72 hours with statistically significant differences between the experimental groups. Conclusions: The intralesional administration of mesenchymal stem cells exercises an antiapoptotic effect in the precocious phases after severe SCI