Efecto neuroprotector de las células madre mesenquimales adultas en las fases iniciales de una lesión traumática de la médula espinal
- Javier Vaquero
- C. Bonilla Horcajo
- L. Otero
- C. Aguayo
- Santiago de Oya Otero
- Mercedes Zurita
ISSN: 1888-6116
Year of publication: 2008
Volume: 19
Issue: 4
Pages: 199-205
Type: Article
More publications in: Trauma
Abstract
Aim: To study if the intralesional administration of adult mesenchymal stem cells modifies the number of apoptotic nervous cells in the early phases after severe spinal cord injury (SCI). Material and methods: Twenty adult Wistar rats were subjected to SCI at dorsal level. Two hours after the lesion, in 10 animals a volume of 10 µl of saline into the lesion were administered. Other 10 animalsreceived 106 bone marrow stromal cells obtained. Five animals of each group were sacrificed at the 8 hours after the lesion and the other 5 animals at the 72 hours. The spinal cord was processed in each animal for conventional histological studies and to identify cells in apoptosis using the marker Mab F7-26 (apostain). The number of cells in apoptosis with appearance of neurons or glial cells was quantified in each group, averaged and subjected to statistical comparisons. Results: In the control group the number of nervous cells expressing apostain was 138 ± 16, at the 8 hours and 82 ± 13 at the 72 hours after lesion. In the treated group the number of nervous cells expressing apostain was 76 ± 11, at the 8 hours, and 57 ± 7, at the 72 hours with statistically significant differences between the experimental groups. Conclusions: The intralesional administration of mesenchymal stem cells exercises an antiapoptotic effect in the precocious phases after severe SCI