Diferenciación neural de células del estroma de médula ósea humana en presencia de líquido cefalorraquídeo

  1. Javier Vaquero
  2. C. Bonilla Horcajo
  3. L. Otero
  4. C. Aguayo
  5. Santiago de Oya Otero
  6. Mercedes Zurita

ISSN: 1888-6116

Year of publication: 2008

Volume: 19

Issue: 4

Pages: 212-217

Type: Article

More publications in: Trauma


Objective: To analyze the influence of cerebrospinal fluid (CSF) upon the neural differentiation of bone marrow stromal cells (BMSCs) induced by chemical agents. Material and methods: A chemical differentiation study was made of human BMSCs, assessing the percentage of the latter that express neural markers at different timepoints following in vitro exposure to chemical neural induction factors. This morphological trans-differentiation process was in turn compared with that obtained after repeating the study under the same conditions but adding 1 ml of CSF to the culture medium, with a brain derived neurotrophic factor (BDNF) concentration of 1.5 pg/ml. Results: Under the experimental conditions of this study, the neurotrophic factors present in CSF did not modify the neural differentiation process of BMSCs mediated by chemical agents in vitro. Conclusions: These findings suggest that when BMSCs are administered within the subarachnoid space, the neurotrophic factors in CSF do not significantly influence the neural differentiation of the cells