Mejora de la capacidad de detección de compuestos empleables en casos de sumisión química en muestras de sangre
- Pablo Prego Meleiro
- Carmen García Ruíz
- Gemma Montalvo García
- Óscar Quintela Jorge
- Germán Ros Magán (coord.)
- Antonio Guerrero Ortega (coord.)
- Cristina Tejedor Martínez (coord.)
- Francisco Pascual Vives (coord.)
- Paloma Ruíz Benito (coord.)
- Vanessa Tabernero Mago (coord.)
Editorial: Editorial Universidad de Alcalá ; Universidad de Alcalá
ISBN: 978-84-16599-48-6
Any de publicació: 2017
Pàgines: 85-92
Congrés: Jornadas de Jóvenes Investigadores de la Universidad de Alcalá (6. 2017. null)
Tipus: Aportació congrés
Resum
The forensic investigation of sexual assaults under chemical submission requires the identification of the chemical substances involved in the organism of the victim. In this investigation, we developed an analytical method for the screening of 104 compounds related to this type of crime in blood samples. We tested several procedures for sample purification, such as solid phase extraction, liquid-liquid extraction and protein precipitation. The analysis were carried out at three levels of concentration using HPlC-MS/MS. We used the double Q-TrAP functionality of the third quadrupole as an ion trap. At high and intermediate concentrations, the three techniques of sample preparation provided similar detection results. However, at the lowest concentration tested, protein precipitation detected a greater number of compounds.