Estudio de la composicion quimica toxicidad actividad antiinlamatoria y antioxidante de chuquiraga spinosa y jungia paniculata (asteraceae)

Résumé

Chuquiraga spinosa and Jungia paniculada (Asteraceae) are used in folk medicine of Nor-Yauyos (Perú) for prostate affections and like antiinflammatory respectively. The hidroalcohólics extracts have not displayed any sign of toxicity to unique dose by oral route in rat, considering that the dl50 is higher than a 2000mg/kg. Both species have shown antiinflammatory activity in vivo, by topi cal admimstration in mouse's ear edema and by oral administration in rat's paw edema. The hidroalcoholic extract of Jungia paniculata has been shown activity against human recombinant synovial phospholipase A2. This mechanism could be the responsible of the antiinflammatory activity in vivo, it has not been found any activity in the generation of NO in RAW 264.7. The methanolic, hidroalcoholic and aqueous extracts of both species have showed antirradi cal activity against dpph, following a slow kinetic and correlated very significantly with the amount of total polyphenols and flavonoids. Jungia paniculata hidroalcoholic extract is the most antioxidant one with an IC50 similar to BHT. in the screening activity against pathogenic fungi of fruits, methanolic and aqueous extracts of Jungia paniculata have showed concentration-activity relationship against Botrytis cinérea. The aqueous extract has al so inhibited the growth of Alternaria altérnate, Penicillium expansum and Rhyzopus stolonifer. in Chuquiraga spinosa it has been identified i soramnetin-3-o-rutinoside, i soramneti n-3-O-glucoside, querceti n-3-0-β-d-glucuronide, kaemferol-3-0-β-d-glucuronide and isoramnetin-3-0-β-d-glucuronide, and in Jungia paniculata api genin-7-O-glucoside, luteolin-7-O-glucoside, api genin and luteolin, none of them has been described until the moment in these genus. The three-glucuronid derivatives isolated have inhibited the superoxide anion, generated by pmns stimulated with TPA and hypoxanthine-xanthine oxidase system.