Mapping and organization of highly-repeated sequences by means of fluorescence in situ hybridization against synaptonemal complex-associated chromatin of rye.

  1. Nieves Cuñado Rodríguez
  2. Jose Barrios Diaz 1
  3. Juan Luis Santos Coloma 1
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Libro:
Some Aspects of Chromosome Structure. RC Sobti, G Obe (eds)

Editorial: Narosa Publishing House, New Delhi

ISBN: 0-7923-7057-0

Ano de publicación: 2002

Páxinas: 11-16

Tipo: Capítulo de libro

DOI: 10.1007/978-94-010-0334-6_2 GOOGLE SCHOLAR lock_openAcceso aberto editor

Obxectivos de Desenvolvemento Sustentable

Resumo

A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescent in situ hybridization has been applied to analyze the location and organization of five different highly-repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin has different properties with regard to anchorage to the SC. While the organization of telomere sequences is maintained in different organisms, species seem to differ with respect to the organization of centromeric satellites.