Validated RP-HPLC Method for Simultaneous Amoxicillin and Meloxicam Determination in Combined Dosage Forms

  1. T. Dao-Thanh 2
  2. Juan José García Rodríguez 1
  3. Covadonga Álvarez Álvarez 23
  4. Paloma de la Torre Iglesias 23
  1. 1 Departamento de Parasitología. Facultad de Farmacia. Universidad Complutense de Madrid
  2. 2 Departamento de Farmacia y Tecnología Farmaceútica. Facultad de Farmacia. Universidad Complutense de Madrid
  3. 3 Instituto de Farmacia Industrial. Facultad de Farmacia. Universidad Complutense de Madrid
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Revista:
Journal of Pharma Research

ISSN: 2319-5622

Año de publicación: 2015

Volumen: 4

Número: 11

Páginas: 355-660

Tipo: Artículo

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Otras publicaciones en: Journal of Pharma Research

Resumen

A simple and sensitive reversed-phase high-performance liquid chromatographic method (RP-HPLC) has been developed and validated for the simultaneous determination of meloxicam (MLX) and sodium amoxicillin (AMX) in their combined dosage forms. Both drugs were separated on a C18 column (250 mm × 4.6 mm, 5 µm). The mobile phase used was acetonitrile: buffer (30:70, v/v) (pH 6), at a flow rate of 1.0 mL/min, with UV detection at 270 nm. The method was validated in the samples concentration ranges of 0.5-15 µg/mL for MLX and 3-50 µg/mL for AMX, where it demonstrated good linearity, accuracy and precision. And the method showed the specificity for the determination of MLX and AMX in the presence of the excipients used in the pharmaceutical formulations and their degradation products under stress conditions. The proposed method can be used for the quality control of formulated products containing both drugs