Caracterización de la formación de P-bodies y de la función de la proteína Pat1 en situaciones de estrés que afectan a la integridad celular de Saccharomyces cerevisiae

Abstract

The fungal cell wall is a dynamic and essential structure whose function is to protect the cell against adverse environmental conditions, ensuring cell viability and survival. The adaptation of yeast to situations that can produce damage to the cell wall is mainly regulated by MAPK Cell Wall Integrity (CWI) pathway, via the MAPK Slt2, which is activated by phosphorylation under these conditions. This activation induces changes in the transcriptional program of the cell to adapt it to stress and survive under adverse conditions. In multiple stress situations, eukaryotic cells, including yeasts, accumulate cytoplasmic aggregates consisting of mRNA and proteins, such as Processing-bodies (PBs). PBs have been associated with mRNA degradation due to elements belonging to the mRNA degradation machinery being abundant in their proteome, and withm RNAs storage sites for subsequent translation. The movement of mRNAs between polysomes and PBs has been described, suggesting their involvement in the regulation of gene expression through the control of mRNA translation. PBs have also been linked to several human diseases, highlighting their biological importance...