VIII Premio Laboratorios Ovejero: Análisis del estrés oxidativo y del inflamasoma NLRPl3 en pacientes con baja respuesta a la estimulación ovárica

  1. Lisa Rancan
  2. Beatriz Linillos Pradillo
  3. Rocío Núñez Calonge
  4. Elena Vara
Revista:
Anales de la Real Academia de Ciencias Veterinarias

Any de publicació: 2021

Volum: 29

Número: 29

Pàgines: 100-124

Tipus: Article

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Resum

Background: A wide range of pathophysiological conditions are associated with fertility issues. Oxidative stress associated with chronic low-grade inflammation has emerged as a plausible key contributor to their pathogenesis. Oxidative stress can activate the NLRP3 inflammasome which can start an inflammatory form of cell death. Low ovarian response (LOR) are patients which are characterized by having a decreased ovarian follicular pool. Although it has been associated to different diseases, an association between oxidative stress and NLRP3 activation with LOR has not been described to date. Hence, this study aimed to investigate the role of the oxidative stress and the NLRP3 inflammasome activation in LOR patients. Methods: A prospective clinical observational study involved 30 donors and 22 patients. Stimulation protocols were the same for all patients. Collection of oocyte-granulose complexes (GCs) and cumulus cells (CCs) was performed during oocyte retrieval. mRNA expression of oxidative markers and inflammasome components were analyzed by qRT-PCR. Non parametric tests were used to identify significant differences between groups and spearmen correlation to asses correlation. Results: A significantly increased mRNA expression of oxidative stress markers as well as inflammasome components and inflammatory cytokines was observed in LOR patients compared to donors. In the same manner, caspase 1, 12 and 18 were also significantly increased in our group of patients in comparison to the donor expression (p<0.0001,p=0.0008 and p=0.0003 respectively). Conclusion: Our results show higher oxidative stress expression and NLRP3 activation in patients with low ovarian reserve compared to donors suggesting an activation of the pathway and its possible involvement in the pathogenesis.

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