The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida albicans
- Hidalgo Vico, Susana 1
- Prieto Prieto, Antonio Daniel 1
- Alonso Monge, Rebeca María Mar 1
- Román González, Elvira 1
- Pla Alonso, Jesús 1
- 1 Departamento de Microbiología y Parasitología, Universidad Complutense de Madrid, Madrid, Spain
ISSN: 2309-608X
Año de publicación: 2021
Volumen: 7
Número: 7
Páginas: 1-14
Tipo: Artículo
Otras publicaciones en: Journal of Fungi
Resumen
Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition WOR1 has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor (WOR1OE) suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration. The WOR1OE cells are deficient in using trehalose and xylose and are unable to use 2C sources, which is consistent with a reduction in the amount of Icl1, the isocitrate lyase enzyme. The icl1Δ/Δ mutants overexpressing WOR1 are deficient in the production of phloxine B positive cells, a main characteristic of opaque cells, a phenotype also observed in mating type hemizygous mtla1Δ icl1Δ/Δ cells, suggesting the involvement of Icl1 in the adaptation to the commensal state. In fact, icl1Δ/Δ cells have reduced fitness in mouse gastrointestinal tract as compared with essentially isogenic heterozygous ICL1/icl1Δ, but overproduction of WOR1 in an icl1Δ/Δ mutant does not restore fitness. These results implicate the glyoxylate shunt in the adaptation to commensalism of C. albicans by mechanisms that are partially independent of WOR1.Keywords: commensalism; glyoxylate cycle; wo switching; fungal pathogenesis; epigenetics; opaque cells; oxidative stress
Información de financiación
Financiadores
-
Ministerio de Economía y Competitividad
- PGC2018-095047-B-I00
-
Comunidad de Madrid
- InGEMICS (B2017/BMD3691)
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