Adequate hydration status promotes a lower concentration of proinflammatory cytikines in healthy adults

Zusammenfassung

Background: Good hydration status (HS) is necessary foran adequate homeostasis of the organism. Cytokines aresecreted mainly by inflammatory leukocytes and act asintercellular mediators.Objetive: Assessing pro and anti-inflammatory cytokinesconcentration in serum and in the aqueous phase of stools(APhS) from healthy adults in function of their HS.Methods: HS data were obtained from 86 healthy adultsof 45-65 years old and BMI ≥18.5-<40 kg/m2. HS wasmeasured by bioelectrical impedance (BIA) with a standarized protocol. Cytokines serum concentrations were determined by multiple ELISAs. Stools were recollected by theparticipants, frozen, and carefully transported to the laboratory where they were stored at -80°C until their determination. Stools were ultra-centrifuged and cytokines weremeasured in APhS with an ultra-sensible cytokines array. Allsamples were analyzed in duplicate.Results: Mean age was 51.2 ± 4.9 years old and BMI was28.2 ± 4.7 kg/m2. The average intake of water from foodsand beverages was not adequate enough (1,411.6 ± 427.4ml/day; 81% consumed less than two-thirds of the recommended intake) however only 89.5% showed an adequateHS and only 10.5% showed clearly dehydration measured byBIA. Volunteers who had good HS had lower values of IFNγ(2.7 ± 2.4 vs 6.4 ± 4.3 pg/ml; p < 0.05) and IL6 serum (5.5 ±13.3 vs 6.4 ± 16.3 pg/ml; p < 0.01) than those who had adehydration status. IL1 from AphS showed lower values inadults with good hydration than those dehydrated (648.3 ±615 vs 1,194 ± 561.2 pg/ml; p < 0.05).Conclusions: Adults with an appropriate HS have a minorconcentration of pro-inflammatory cytokines in serum andin APhS than adults who showed a dehydration status. Morestudies are needed in order to corroborate these results.Key words: Body water. Hydration. Dehydration. Cytokinesand bioelectrical impedance analysis.