Estudio de la infección por virus del papiloma humano en lesiones bucales y su relación con la carcinogénesis

  1. A Bascones Martínez 1
  2. JA García Núñez 1
  3. MJ Alonso Martín 2
  4. F Gómez Aguado
  5. M Roldán Contreras
  6. F Llanes Méndez 3
  7. A Picazo Talavera
  8. MT Comero Pindado
  9. GC Esparza Gómez 1
  10. R Cerero Lapiedra 1
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

  2. 2 A. Patológica Carlos III
  3. 3 Hospital de San Carlos
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Journal:
RCOE: Revista del Ilustre Consejo General de Colegios de Odontólogos y Estomatólogos de España

Year of publication: 1996

Volume: 1

Issue: 6

Pages: 437-445

Type: Article

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Abstract

55 biopsies from the oral cavity were studied, diagnosed as fibroma, papilloma erosive lichen, leucoplakia and carcinoma, appling inmunohistochemical techniques with an anti-HPV policlonal antibody (human pailloma virus), and in situ hybridization with a probe of generic ADN specific of HPV and 3 specific probes of 6/11, 16/18 and 31/33/35 types of HPV. It is also studied the relationship between these viral types and the proliferation degree of the infected epithelium using the MIB 1 antibody (anti Ki-67). Seven biopsies (12,7%) were positive for HPV by inmunohistochemistry, while in 20 biopsies (36,4%) genetic secuencies of HPV were detected with in situ hybridization. By lesions, best result was obtained in papillomas, with 66,7% positive. In benign lesions were detected similar proportions of viral types, while in carcinomas were detected only intermediate and high risk types. It was observed with the MIR 1 antibody that in 84% of non invading lesions practically all the basal layer cells of the epithelium were in cycle. On the contrary, only in two leucoplakias were detected positive MIB 1 cells in the upper third of the epithelium. No significant estatistical correlation has been found among the detected viral type in one lesion and the proportion of MIB 1 positive epithelial cells. We find that in situ hybridization is a more sensitive technique than the inmunohistochemistry for studing these oral lesions and that the HPV can play an etiologic role in benign, premalign and malign lesions. The MIB 1 antibody may be useful in the pronostic value of the premalign lesions.

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