Suitable method for simultaneous and specific detection of maize (zea mays l.) andgenetically modified soybean (glycine max l.) in animal feeds
- A. Gómez Garay 1
- Garrido Mayas 1
- Vergara García, G. 1
- 1 Biosafety Laboratory, IMIDRA, El Encín A-2 km 38.200. 28800 Alcalá de Henares, Spain
ISSN: 1230-1388
Année de publication: 2009
Volumen: 18
Pages: 162-172
Type: Article
D'autres publications dans: Journal of Animal and Feed Sciences
Objectifs de Développement Durable
Résumé
Present method allows a simple and rapid identifi cation of maize and specifi c transgenic soyabean event in raw materials and processed feed samples. The method combines two aspects: suitable DNA purifi cation and amplifi cation by means of multiplex RT-PCR. Effi ciency and accuracy of this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μl for one of the species and of 1 ng/μl for the other one. The checking of the method has been realized by means of RT-PCR using the specifi c probes for each one of the systems by means of comparison with certifi ed reference materials. The obtained results showed that with this method it is possible to obtain, in a shorter time, quality values for both: identifi cation and quantifi cation of GMOs, in raw and processed samples.