TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers

  1. Rico-San Román, L.
  2. Hänggeli, K.P.A.
  3. Hemphill, A.
  4. Horcajo, P.
  5. Collantes-Fernández, E.
  6. Ortega-Mora, L.M.
  7. Boubaker, G.
Journal:
Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988

Year of publication: 2024

Volume: 14

Type: Article

DOI: 10.3389/FCIMB.2024.1419209 GOOGLE SCHOLAR lock_openOpen access editor