Cuantificación de DNA antiguo por real time PCR

  1. D. Turbón 1
  2. E. Fernández 1
  3. E. Arroyo 2
  4. A. Pérez Pérez 1
  5. L. Cornudella 3
  1. 1 Universitat de Barcelona
    info

    Universitat de Barcelona

    Barcelona, España

    ROR https://ror.org/021018s57

  2. 2 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

  3. 3 IBMB-CSIC
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Libro:
Actas XIII Congreso SEAB. Biología de Poblaciones Humanas: Diversidad, tiempo, espacio
  1. José Enrique Egocheaga (ed. lit.)
  2. M. J. Sierra (comp.)

Editorial: Sociedad Española de Antropología Física, SEAF

ISBN: 84-609-1519-0

Ano de publicación: 2004

Páxinas: 633-639

Congreso: Congreso Español de Antropología Biológica (13. 2003. Oviedo)

Tipo: Achega congreso

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Resumo

We developed an essay for estimating the amount of 150bp fragments of Hypervariable I region in mtDNA through Real Time PCR (RT-PCR). The method allos a successful detection of an amount between 3.58 X 10⁻⁵ and 3.5 ng (10⁴ - 10⁹ copies) of template DNA. We analyzed several samples form different ages and archaeological sites (Paleolithic, Neolithic, Bronze Age and Calcolitic). Most of the samples yielded amounts larger than 10⁴ copies. This can indicate a good state of preservation and shows that Real Time PCR quantification is a reliable method for the detection of ancient human DNA. The method does not overestimate the DNA amount because of the presence of microbial DNA as other methods, i.e. spectophotometry, do. Our results show no relationship between the preserved amount of DNA and the age of the sample.

Fonte de datos: Dialnet