JUAN MANUEL
BOLÍVAR BOLÍVAR
Postdoctoral UCM
Instituto de Catálisis y Petroleoquímica
Madrid, EspañaPublicaciones en colaboración con investigadores/as de Instituto de Catálisis y Petroleoquímica (27)
2020
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Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes
Methods in Molecular Biology (Humana Press Inc.), pp. 93-107
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One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized “Native Enzymes”
Methods in Molecular Biology (Humana Press Inc.), pp. 83-92
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The Science of Enzyme Immobilization
Methods in Molecular Biology (Humana Press Inc.), pp. 1-26
2013
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Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports
Methods in Molecular Biology, Vol. 1051, pp. 73-88
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Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups
Methods in Molecular Biology, Vol. 1051, pp. 59-71
2012
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Characterization and further stabilization of a new anti-prelog specific alcohol dehydrogenase from Thermus thermophilus HB27 for asymmetric reduction of carbonyl compounds
Bioresource Technology, Vol. 103, Núm. 1, pp. 343-350
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Stabilization of a highly active but unstable alcohol dehydrogenase from yeast using immobilization and post-immobilization techniques
Process Biochemistry, Vol. 47, Núm. 5, pp. 679-686
2011
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Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate
Journal of Biotechnology, Vol. 155, Núm. 4, pp. 412-420
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New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme
BMC Biotechnology, Vol. 11
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Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum
Food Chemistry, Vol. 128, Núm. 1, pp. 214-217
2010
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Complete reactivation of immobilized derivatives of a trimeric glutamate dehydrogenase from Thermus thermophillus
Process Biochemistry, Vol. 45, Núm. 1, pp. 107-113
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Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports
Process Biochemistry, Vol. 45, Núm. 10, pp. 1692-1698
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Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports
Biomacromolecules, Vol. 11, Núm. 11, pp. 3112-3117
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Selective adsorption of small proteins on large-pore anion exchangers coated with medium size proteins
Colloids and Surfaces B: Biointerfaces, Vol. 78, Núm. 1, pp. 140-145
2009
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Coating of soluble and immobilized enzymes with ionic polymers: Full stabilization of the quaternary structure of multimeric enzymes
Biomacromolecules
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Effect of chain length on the activity of free and immobilized alcohol dehydrogenase towards aliphatic alcohols
Enzyme and Microbial Technology, Vol. 44, Núm. 3, pp. 135-138
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Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers
Journal of Biotechnology, Vol. 144, Núm. 2, pp. 113-119
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Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus
Enzyme and Microbial Technology, Vol. 44, Núm. 6-7, pp. 386-393
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Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization
Journal of Molecular Catalysis B: Enzymatic, Vol. 58, Núm. 1-4, pp. 158-163
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Purification, immobilization and stabilization of a highly enantioselective alcohol dehydrogenase from Thermus thermophilus HB27 cloned in E. coli
Process Biochemistry, Vol. 44, Núm. 9, pp. 1004-1012