JOSÉ MIGUEL
MANCHEÑO GÓMEZ
Investigador hasta 2008
FRANCISCO JAVIER
LACADENA GARCÍA-GALLO
Profesor titular de universidad
Publicaciones en las que colabora con FRANCISCO JAVIER LACADENA GARCÍA-GALLO (21)
2001
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Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin α-sarcin
European Journal of Biochemistry, Vol. 268, Núm. 23, pp. 6190-6196
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Involvement of the amino-terminal β-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity
Protein Science, Vol. 10, Núm. 8, pp. 1658-1668
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RNase U2 and α-sarcin: A study of relationships
Methods in Enzymology (Academic Press Inc.), pp. 335-351
2000
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Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin α-Sarcin
Proteins: Structure, Function and Genetics, Vol. 41, Núm. 3, pp. 350-361
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Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus
Protein Expression and Purification, Vol. 18, Núm. 1, pp. 71-76
1999
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Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin α-sarcin
Proteins: Structure, Function and Genetics, Vol. 37, Núm. 3, pp. 474-484
1998
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Aggregation and liquid-mixing of dimyristorylphosphatidil-serine vesicles induced by the cytotoxin [Alpha]-sarcin
Revista de la Real Academia de Ciencias Exactas, Físicas y Naturales, Vol. 92, Núm. 2, pp. 185-194
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Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes
Molecular Membrane Biology, Vol. 15, Núm. 3, pp. 141-144
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Secretion of recombinant pro- and mature fungal α-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: The Lys-Arg motif is required for maturation
Protein Expression and Purification, Vol. 12, Núm. 3, pp. 315-322
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The cytotoxin α-sarcin behaves as a cyclizing ribonuclease
FEBS Letters, Vol. 424, Núm. 1-2, pp. 46-48
1997
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Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus
Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1340, Núm. 1, pp. 81-87
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Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818
Archives of Biochemistry and Biophysics, Vol. 343, Núm. 2, pp. 188-193
1995
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Characterization of the antifungal protein secreted by the mould aspergillus giganteus
Archives of Biochemistry and Biophysics, Vol. 324, Núm. 2, pp. 273-281
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Escherichia coli JA221 can suppress the UAG stop signal
Letters in Applied Microbiology, Vol. 21, Núm. 2, pp. 96-98
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Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116–139th sequence region of the cytotoxic protein alpha-sarcin
Biophysical Journal, Vol. 68, Núm. 6, pp. 2387-2395
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Spectroscopic characterization of the alkylated α-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction
Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1252, Núm. 1, pp. 43-52
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Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein α-sarcin
Biochemical Journal, Vol. 309, Núm. 2, pp. 581-586
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Thermal unfolding of the cytotoxin α-sarcin: phospholipid binding induces destabilization of the protein structure
Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1252, Núm. 1, pp. 126-134
1994
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Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements
Biophysical Journal, Vol. 67, Núm. 3, pp. 1117-1125
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Overproduction and purification of biologically active native fungal α-sarcin in Escherichia coli
Gene, Vol. 142, Núm. 1, pp. 147-151