Bioquímica y Biología Molecular
Departamento
JUAN MANUEL
BOLÍVAR BOLÍVAR
Investigador postdoctoral
Publicaciones en las que colabora con JUAN MANUEL BOLÍVAR BOLÍVAR (24)
2024
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Designing tailor-made steric matters to improve the immobilized ficin specificity for small versus large proteins
Journal of Biotechnology, Vol. 395, pp. 12-21
2023
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Glyoxyl-ficin: An example where a more intense multipoint covalent attachment may decrease enzyme stability.
Process Biochemistry, Vol. 132, pp. 289-296
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Synergy of Ion Exchange and Covalent Reaction: Immobilization of Penicillin G Acylase on Heterofunctional Amino-Vinyl Sulfone Agarose
Catalysts, Vol. 13, Núm. 1
2022
2020
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Erratum: Correction to: Immobilization of Enzymes and Cells (Methods in molecular biology (Clifton, N.J.) (2020) 2100 (189-198))
Methods in molecular biology (Clifton, N.J.)
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Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes
Methods in Molecular Biology (Humana Press Inc.), pp. 93-107
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One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized “Native Enzymes”
Methods in Molecular Biology (Humana Press Inc.), pp. 83-92
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Preface
Methods in Molecular Biology
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The Science of Enzyme Immobilization
Methods in Molecular Biology (Humana Press Inc.), pp. 1-26
2015
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Immobilization of proteins on highly activated glyoxyl supports: Dramatic increase of the enzyme stability via multipoint immobilization on pre-existing carriers
Current Organic Chemistry, Vol. 19, Núm. 17, pp. 1719-1731
2013
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Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports
Methods in Molecular Biology, Vol. 1051, pp. 73-88
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Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups
Methods in Molecular Biology, Vol. 1051, pp. 59-71
2012
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Characterization and further stabilization of a new anti-prelog specific alcohol dehydrogenase from Thermus thermophilus HB27 for asymmetric reduction of carbonyl compounds
Bioresource Technology, Vol. 103, Núm. 1, pp. 343-350
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Stabilization of a highly active but unstable alcohol dehydrogenase from yeast using immobilization and post-immobilization techniques
Process Biochemistry, Vol. 47, Núm. 5, pp. 679-686
2011
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New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme
BMC Biotechnology, Vol. 11
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Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum
Food Chemistry, Vol. 128, Núm. 1, pp. 214-217
2010
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Complete reactivation of immobilized derivatives of a trimeric glutamate dehydrogenase from Thermus thermophillus
Process Biochemistry, Vol. 45, Núm. 1, pp. 107-113
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Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports
Biomacromolecules, Vol. 11, Núm. 11, pp. 3112-3117
2009
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Coating of soluble and immobilized enzymes with ionic polymers: Full stabilization of the quaternary structure of multimeric enzymes
Biomacromolecules
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Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization
Journal of Molecular Catalysis B: Enzymatic, Vol. 58, Núm. 1-4, pp. 158-163