Functional differences between dorsal and ventral hippocampus revealed with agnor staining .

  1. González Pardo, Héctor 1
  2. García Moreno, Luis Miguel
  3. Arias Pérez, Jorge Luis 1
  4. Cimadevilla Redondo, José Manuel 1
  1. 1 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

Revista:
Psicothema

ISSN: 0214-9915

Año de publicación: 2000

Volumen: 12

Número: 2

Páginas: 293-295

Tipo: Artículo

Otras publicaciones en: Psicothema

Resumen

Recientemente se han encontrado diferencias en cuanto a la actividad neural de la formación hipocámpica en sus zonas dorsal y ventral. Estas diferencias podrían jugar un importante papel en cuanto a la implicación diferencial de estas zonas en el aprendizaje y la memoria. Aplicamos aquí la técnica histológica de impregnación argéntica de las regiones organizadoras del nucleolo (AgNORs) con el fin de analizar la actividad biosintética de las areas hipocampales CA1 y CA3 en las zonas dorsal y ventral de ratas Wistar. Se cuantificaron el número y área media de los AgNORs presentes en sus neuronas, que son indicadores indirectos de la actividad de síntesis proteica. Los resultados muestran una actividad biosintética significativamente mayor en la región dorsal de ambas áreas estudiadas, que concuerda con estudios anteriores y se discuten sus implicaciones funcionales.

Referencias bibliográficas

  • Bannerman, D.M., Yee, B.K., Good, M.A., Heupel, M.J., Iversen, S.D. and Rawlins, J.N.P. (1999) Double dissociation of function within the hippocampus: a comparison of dorsal, ventral, and complete hippocampal cytotoxic lesions. Behavioral Neuroscience 113(6): 1170-1188.
  • Bruinink, A. and Bishoff, S. (1993) Dopamine D2 receptors are unevenly distributed in the rat hippocampus and are modulated differently than in striatum. European Journal of Pharmacology 245(2):157-164.
  • Crocker, J. (1996) Correlation between NOR sizes and numbers in nonHodgkin’s lymphomas. Journal of Clinical Pathology 49: 8-11.
  • Derenzini, M., Farabegoli, F. and Treré, D. (1992) Relationship between interphase AgNOR distribution and nucleolar size in cancer cells. Journal of Histochemistry 24: 951-956.
  • Eichenbaum, H., Dudchenko, P., Wood, E., Shapiro, M. and Tanila, H. (1999) The hippocampus, memory, and place cells: is it spatial memory or a memory space? Neuron 23(2): 209-226.
  • Fushiki, S., Kinoshita, C., Tsutsumi, Y. and Nishizawa, Y. (1995) A combined staining method for argyrophilic nucleolar organizer regions and for glial fibrillary acidic protein in astrocytes of human brain. Acta Histochemica and Cytochemica 28(6): 533-538.
  • García-Moreno, L.M., Cimadevilla, J.M., González-Pardo, H., Zahonero, M.C. and Arias, J.L. (1997) NOR activity in hippocampal areas during the postnatal development and ageing. Mechanisms of Ageing and De velopment 97:173-181.
  • García Moreno, L.M., Santín, L.J., Rubio, S., González-Pardo, H. and Arias, J.L. (1993) Effects of ethanol and diazepam on AgNOR neuronal activity in the medial mammillary nucleus. Psicothema 5(1): 125-134.
  • González-González, S., Díaz, F., Vallejo, G. and Arias, J.L. (1996) Functional sexual differences in rat mammillary bodies: a quantitative AgNOR study. Brain Research 736:1-6.
  • Jung, M.W., Wiener, S.I. and McNaughton, B.L. (1994) Comparison of spatial firing characteristics of units in dorsal and ventral hippocampus of the rat. Journal of Neuroscience 14(12): 7347-7356.
  • Lafarga, M., Andrés, M.A., Fernández Viadero, C., Villegas, J. and Berciano, M.T. (1995) Number of nucleoli and coiled bodies and distribution of fibrillar centres in differentiating Purkinje neurons of chick and rat cerebellum. Anatomy and Embriology 191(4): 359-367.
  • Levin, E.D., Channelle Christopher, N., Weaver, T., Moore, J. And Brucato, F. (1999) Ventral hippocampal ibotenic acid lesions block chronic nicotine-induced spatial working memory improvement in rats. Cognitive Brain Research 7: 405-410.
  • Lorenzini, C.G.A., Baldi, E., Bucherelli, C., Sacchetti, B and Tassoni, G. (1997) Role of ventral hippocampus in acquisition, consolidation and retrieval of rat’s passive avoidance response memory trace. Brain Research 768: 242-248.
  • Moser, E., Moser, M.B. and Andersen, P. (1993) Spatial learning impairment parallels the magnitude of dorsal hippocampal lesions, but is hardly present following ventral lesions. Journal of Neuroscience 13(9):3916-3925.
  • O’Keefe, J., Burgess, N., Donnett, J.G., Jeffery, K.J. and Maguire, E.A. (1998) Place cells, navigational accuracy, and the human hippocampus. Philosophical Transactions from the Royal Society of London B, 353(1373): 1333-1340.
  • Otani, S., Roisin-Lallemand, M.P. and Ben-Ari, Y. (1992) Enhancement of extracellular protein concentrations during long-term potentiation in the rat hippocampal slice. Neuroscience47(2): 265-272.
  • Ploton, D., Visseaux-Coletto, B., Canellas, J.C., Bourzat, C., Adnet, J.J., Lechki, C. and Bonnet, N. (1992) Semiautomatic quantification of silver-stained nucleolar organizer regions in tissue sections and cellular smears. Analytical and Quantitative Cytology and Histology 14(1):14- 22.
  • Richmond, M.A., Yee, B.K., Pouzet, B., Veenman, L., Rawlins, J.N.P., Feldon, J. and Bannerman, D.M. (1999) Dissociating context and space within the hippocampus: effects of complete,dorsal, and ventral excitotoxic hippocampal lesions on conditioned freezing and spatial learning. Behavioral Neuroscience 113(6): 1189-1203.
  • Room, P. and Groenewegen, H.J. (1986) Connections of the parahippocampal cortex. I cortical afferents. Journal of Comparative Neurology 209: 69-78.
  • Scoville, W.B. and Milner, B. (1957) Loss of recent memory after bilateral hippocampal lesions. Journal of Neurology, Neurosurgery and Psychiatry 20: 11-2.
  • Stanton, P.K. and Sarvey, J.M. (1984) Blockade of long-term potentiation in rat hippocampal CA1 region by inhibitors of protein synthesis. Journal of Neuroscience 4(12): 3080-3088.
  • Witter, M.P., Van Hoesen, G.W. and Amaral, D.G. (1989) Topographical organisation of the entorhinal projection to the dentate gyrus of the monkey. Journal of Neuroscience 54: 5-9.