Estudio comparativo de la toxicidad del metilmercurio, cadmio (II) y cromo (VI) en cultivos primarios de neuronas y astrocitos de rata

  1. Uroz Martínez, V. 2
  2. Anadón Baselga, María José 2
  3. Capó Martí, Miguel Andrés 1
  1. 1 Departamento de Toxicología Y Farmacología, Facultad de Veterinaria, Universidad Complutense de Madrid
  2. 2 Departamento de Toxicología Y Legislación Sanitaria, Facultad de Medicina, Universidad Complutense de Madrid
Journal:
Revista de toxicología

ISSN: 0212-7113

Year of publication: 2008

Volume: 25

Issue: 1-3

Pages: 56-60

Type: Article

SCOPUS: 2-s2.0-58149147010 DIALNET GOOGLE SCHOLAR

More publications in: Revista de toxicología

Abstract

This study aims to compare letal concentration 50 (LC , toxic dose causing death of 50% of the cells) of methylmercury, 50 cadmium (II) and chromium (VI) in primary cultures of rat cortex. Cerebral hemispheres of rat were used to prepare the primary cultures. The neuronal primary cultures were performed from embryonic Wistar rats of 14-16 days of gestation in Dulbecco's modification of Eagle's medium. Astrocytes primary cultures were prepared with neonatal Wistar rats in Waymouth´s medium. Methylene blue and trypan blue staining were used to assess neuronal death with cytometry. LC for both neurons and astrocytes were 50 calculated by means of Reed-Muench method. The main results were: -6 LC in 24-hour culture of neurons was 5 x 10 M for methylmercury, 50 -6 -6 3.7 x 10 M for cadmium (II) and 5.34 x 10 M for chromium (VI). -5 LC in 24-hour cultures of astrocytes was of 1.46 x 10 M for 50 -5 -5 methylmercury, 3.73 x 10 M for cadmium (II) and 2.46 x 10 M for chromium (VI). These results demonstrate that astrocytes are approximately ten times more resistant than neurons for the three toxic compounds.

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