Cementos de silicato de calcioevaluación de la toxicidad y de la respuesta inflamatoria

  1. Yolanda Freire Mancebo 1
  2. Mar Larrosa Pérez 1
  3. Isabel Esteban Martínez
  4. Beatriz Palma White
  5. Rafael Cisneros Cabello 1
  1. 1 Universidad Europea de Madrid
    info

    Universidad Europea de Madrid

    Madrid, España

    ROR https://ror.org/04dp46240

Aldizkaria:
Científica dental: Revista científica de formación continuada

ISSN: 1697-6398,  1697-641X

Argitalpen urtea: 2021

Alea: 18

Zenbakia: 3

Orrialdeak: 8-14

Mota: Artikulua

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Beste argitalpen batzuk: Científica dental: Revista científica de formación continuada

Laburpena

Biocompatibility of sealers is a feature to be taken into account as it might influence the outcome of the treatment. The aim of this study was to analyze the cytotoxicity of the silicate-based root canal sealers BioRoot™ RCS and Nanoceramic Sealer™and the epoxy-resin based sealer AH Plus Jet™, as well as their associated inflammatory response in human fibroblast. Thirty-six human teeth were selected, prepared, filled with the selected sealers and put in contact with culture medium to obtain extracts at 24, 48 and 72 hours. Then, the extracts were cultured with the L-132 fibroblast cell line. Cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay. Inflammatory response was analyzed by measuring IL-6 levels determined by ELISA and nitrite levels (fluorescence assay). All sealers showed a certain degree of cytotoxicity during the evaluation time, while only the BioRoot™ RCS triggered a pro-inflammatory response. Further research is needed to assess other aspects of biocompatibility of silicate-based root canal sealers.

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