Alternaria spp. and their mycotoxins in tomatoes. A scientific approach from field to food industry
- Estiarte Piñol, Núria
- A. Crespo-Sempere Director
- Antonio J. Ramos Girona Director
Defence university: Universitat de Lleida
Fecha de defensa: 12 July 2016
- Inmaculada Viñas Almenar Chair
- Belén Patiño Alvarez Secretary
- Naresh Magan Committee member
Type: Thesis
Abstract
ABSTRACT As a result of the publication of the EFSA’S scientific report, entitled “Scientific opinion on the risks for animal and public health related to the presence of Alternaria toxins in feed and food”, arose the idea of developing this Doctoral Thesis. The first steps were to assess the presence of viable Alternaria spp. using the PMA-qPCR technique and, additionally, analyze the occurrence of two Alternaria mycotoxins (AOH and AME). A second approach to the problem was to focus the study in analyzing two components of the Velvet family (VeA and LaeA) and, at the same time, the polyamine metabolism in order to find new targets useful to design new fungicides. Secondly, in a postharvest context, effects of 1-methylcyclopropene were analyzed on A. alternata infecting tomatoes in relation to fungal growth and mycotoxin production. Finally, the stability of AOH and AME along the industrial process of tomato derivate products was studied.