Preliminary report on cross-species microsatellite amplification for bumblebee biodiversity and conservation studies

  1. Cejas Acuña, D. 1
  2. Ornosa Serrano, C. 2
  3. Muñoz Gabaldón, I. 1
  4. De la Rúa Tarín, P. 1
  1. 1 Universidad de Murcia. Murcia. España.
  2. 2 Universidad Complutense de Madrid. Madrid. España.
Journal:
Archivos de zootecnia

ISSN: 0004-0592,  1885-4494

Year of publication: 2019

Volume: 68

Issue: 263

Pages: 428-432

Type: Article

DOI: 10.21071/AZ.V68I263.4204 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Archivos de zootecnia

Abstract

The Iberian Peninsula holds a high diversity of bumblebees but there is a general lack of information about their biodiversity in this area. To overcome this and facilitate conservation studies, we present two novel multiplex assays for the amplification of six and five microsatellite loci respectively. Both assays successfully amplified for most of the studied species in the Iberian populations. Sibling workers and population genetic parameters were analysed in the managed species B. terrestris and in the wild species B. monticola and B. mesomelas, demonstrating the capability of these multiplex assays for biodiversity studies of both managed and wild bumblebee species.

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Funding information

Sampling permissions were obtained from the corresponding authorities (Parques Nacionales de Aigüestortes i Estany de Sant Maurici, Sierra de Gua-darrama, Ordesa y Monte Perdido, Sierra Nevada). We thank Dr. Stefan Wolf, Ana Asensio and Nuria Blasco for their laboratory contributions, and Dr. Laura Jara and Dr. Carlos Ruiz for their advice during the analyses. This work was funded by the projects E-RTA2014-00003-C03 (Spanish National Institute for Agricultural and Food Research and Technology, and European Regional Development Fund) and 19908/GERM/2015 of Regional Excellence (Seneca Foundation, CARM). IM is supported by a Saavedra Fajardo fellowship (20036/ SF/16) from the Seneca Foundation (CARM).

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