Wildlife as a Sentinel for Pathogen Introduction in Nonendemic Areas: First Detection of <i>Leishmania tropica</i> in Wildlife in Spain

  1. Azami-Conesa, Iris 23
  2. Matas Méndez, Pablo 1
  3. Pérez-Moreno, Paula 2
  4. Carrión, Javier 2
  5. Alunda, José María 2
  6. Mateo Barrientos, Marta 2
  7. Gómez-Muñoz, María Teresa 2
  1. 1 Universidad Alfonso X el Sabio
    info

    Universidad Alfonso X el Sabio

    Villanueva de la Cañada, España

    ROR https://ror.org/054ewwr15

  2. 2 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

  3. 3 Universidad Europea de Madrid
    info

    Universidad Europea de Madrid

    Madrid, España

    ROR https://ror.org/04dp46240

Erakutsi afiliazioak +
Aldizkaria:
Transboundary and Emerging Diseases

ISSN: 1865-1674,  1865-1682

Argitalpen urtea: 2024

Urtea: 2024

Zenbakia: 1

Mota: Artikulua

DOI: 10.1155/2024/8259712 GOOGLE SCHOLAR lock_openSarbide irekia editor

Beste argitalpen batzuk: Transboundary and Emerging Diseases

Laburpena

Leishmaniasis is a chronic global arthropod-borne zoonotic disease produced by several species of Leishmania with cutaneous, mucocutaneous, and visceral clinical manifestations. In Spain, only Leishmania infantum has been reported so far, although other species of Leishmania, such as L. tropica and L. major, are present in surrounding countries. The aim of this work is to analyze the occurrence of Leishmania spp. infection in European wildcats (Felis silvestris) as sentinels, including their genotypic characterization. Necropsies of 18 road-killed wildcats were conducted. Samples of ear skin and spleen were taken for DNA isolation and PCR of the highly sensitive SSU-rDNA target. Subsequent PCR tests were performed using more specific targets for the determination of Leishmania species: hsp70 and ITS1. Positive samples were sequenced, and phylogenetic trees were constructed. Seven wildcats were found positive for Leishmania spp. Based on the hsp70 and ITS1 sequences, an animal was found to be infected only with L. tropica in ear skin samples, while two cats were found to be infected with L. infantum in both the ear skin and the spleen. In one animal, a clear sequence of L. infantum ITS1 and a sequence of L. tropica hsp70 were obtained from the ear skin. Since hsp70 and ITS1 sequencing was not possible in three cats, the species of Leishmania infecting them was not determined. This is the first report of autochthonous infection with L. tropica in the Iberian Peninsula. Health care professionals, including physicians, dermatologists, and veterinarians, must be aware of this for a correct diagnosis, treatment, and management of possible coinfections.

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Erreferentzia bibliografikoak

  • WHO: health-topics/leishmaniasis https://www.who.int/health-topics/leishmaniasis#tab=tab_1%5bInternet%5d.WHO:health-topics/leishmaniasis 2024.
  • 10.1371/journal.pntd.0004349
  • WHO: neglected diseases leishmaniasis 2023 https://apps.who.int/neglected_diseases/ntddata/leishmaniasis/leishmaniasis.html.
  • 10.1186/s13071-020-04080-8
  • 10.1111/tmi.12877
  • 10.3390/microorganisms9051101
  • 10.3390/pathogens10030307
  • 10.1016/j.prevetmed.2013.12.001
  • 10.1016/j.vetpar.2018.03.015
  • 10.1016/j.vetpar.2018.05.012
  • 10.1098/rsos.190334
  • BerriatuaE. Pérez-CutillasP. González VidalA. andBriëtO. The spatial relationship between the presence and absence ofLeishmaniaspp. and leishmaniasis and phlebotomine sand fly vectors in Europe and neighbouring countries 2023 European Centre for Disease Prevention and Control https://www.ecdc.europa.eu.
  • 10.1186/s13071-020-04081-7
  • 10.1016/j.meegid.2019.104069
  • 10.1016/0035-9203(93)90482-6
  • 10.1016/j.actatropica.2017.05.016
  • 10.1016/j.crpvbd.2023.100150
  • European centre for disease prevention and control and European food safety authority 2023 Phlebotomine sandflies maps [internet]. Stockholm: ECDC; 2022https://ecdc.europa.eu/en/disease-vectors/surveillance-and-disease-data/phlebotomine-maps [Internet].
  • 10.1016/S0035-9203(02)90074-X
  • 10.3390/microorganisms9071531
  • 10.1186/s13071-017-2398-8
  • 10.2807/1560-7917.es2013.18.30.20543
  • 10.1093/molbev/msab120
  • 10.1093/oxfordjournals.molbev.a040023
  • Win Epi Working in epidemiology 2024 Ignacio de Blas. [Internet] Accesed on January 9thhttp://www.winepi.net/.
  • López-MartínJ. M. GarcíaF. J. SuchA. VirgósE. LozanoJ. DuarteJ. andEspañaA. J. Atlas y Libro Rojo de los Mamíferos Terrestres de España 2007 Dirección General para la Biodiversidad -SECEM-SECEMU Madrid. [Internet]. [cited 2023 Dec 6]. Available from:https://www.miteco.gob.es/es/biodiversidad/temas/inventarios-nacionales/inventario-especies-terrestres/inventario-nacional-de-biodiversidad/ieet_mamif_atlas.html.
  • 10.1017/S0031182013000942
  • 10.1111/tmi.12686
  • 10.1016/j.meegid.2021.104932
  • 10.1371/journal.pntd.0010009
  • 10.1016/j.ijpara.2018.12.009
  • 10.1016/j.ijpara.2019.05.009
  • 10.1186/s12879-018-3667-7
  • 10.1111/tbed.14107
  • 10.1111/j.1365-2915.2012.01034.x
  • 10.1111/jdv.14434
  • 10.1111/tbed.12914
  • 10.1186/s13071-022-05272-0
  • 10.1016/j.exppara.2016.05.011
  • 10.1016/j.trstmh.2005.07.011
  • 10.1016/j.actatropica.2019.02.016
  • 10.1186/s12917-015-0392-y
  • 10.1016/j.meegid.2018.05.029
  • 10.1128/JCM.01469-12
  • 10.4269/ajtmh.17-0490
  • 10.1016/j.celrep.2020.03.071
  • 10.1016/j.meegid.2011.04.026
  • 10.1016/j.ijpara.2006.06.019
  • 10.1016/j.ijid.2012.05.702
  • 10.1128/mbio.00995-24
  • Azami-ConesaI. Matas MéndezP. Pérez-MorenoP. CarriónJ. AlundaJ. M. Mateo BarrientosM. andGómez-MuñozM. T. Wildlife as a sentinel for pathogen introduction in nonendemic areas: first detection ofLeishmania tropica in wildlife in Spain 2024 BiorXiv.