Identificación de carnes procedentes de aves de caza y de la avicultura alternativa mediante una técnica de PCR en tiempo real

  1. María Rojas Diéguez 1
  2. Isabel González Alonso 1
  3. Rosario Martín de Santos 1
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Revista:
Revista complutense de ciencias veterinarias

ISSN: 1988-2688

Ano de publicación: 2009

Volume: 3

Número: 2

Páxinas: 79-89

Tipo: Artigo

DIALNET GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Revista complutense de ciencias veterinarias

Resumo

Real-time polymerase chain reaction (PCR) based on TaqMan probes has been applied to the identification of meat from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp), guinea fowl (Numida meleagris), pigeon (Columba spp), Eurasian woodcock (Scolopax rusticola), and song thrush (Turdus philomelos). The method combines the use of species-specific primers and TaqMan probes that amplify small fragments (<200 base pairs) of the mitochondrial 12S rRNA gene, and an endogenous control primer pair that amplifies a 141 bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. Analysis of experimental raw and heat-treated binary mixtures as well as of commercial meat products from the target species, demonstrated the suitability of the assay for the detection of the target DNAs. Key words: game birds, 12S rRNA gene, 18S rRNA gene, real-time PCR, TaqMan probes

Fonte de datos: Dialnet