Efecto de las células oviductales y del cumulus oophorus sobre diferentes parámetros biológicos relacionados con la fecundación in vitro en la especie porcina.
- Romar Andrés, Raquel
- María Pilar Coy Fuster Director
- Salvador Ruiz López Director
Defence university: Universidad de Murcia
Fecha de defensa: 24 January 2001
- Ronald H. F. Hunter Chair
- Emilio Gómez de la Concha Secretary
- María Teresa Paramio Nieto Committee member
- Detlef Rath Committee member
- Raúl Sánchez Committee member
Type: Thesis
Abstract
The objective of this experimental work was to study the effect of porcine epithelial oviductal cells and cumulus cells on several parameters related to in vitro fertilization in pigs. Studied variables were rate of penetration, mean number of sperm cells per penetrated oocyte, rate of male pronuclear formation, cleavage rate at 48h postinsemination, blastocyst formation rate, mean number of cells per blastocyst, resistance of zona pellucida to pronase digestion and density and distribution of cortical granules in the different experimental groups.